Göde Funda, Pala Ibrahim, Yıldırım Fatma, Abdi Zahra
IVF Center, Izmir University of Economics Medical Point Hospital, Izmir, Turkey.
IVF Center, Izmir Medical Point Hospital, Izmir, Turkey.
Gynecol Endocrinol. 2025 Dec;41(1):2515125. doi: 10.1080/09513590.2025.2515125. Epub 2025 Jun 9.
To determine rates of fertilization, good-quality day 3 embryos, and blastocyst progression in sibling oocytes of the same patients undergoing piezo and calcium ionophore (CaI) activation. The retrospective cohort study included couples undergoing assisted reproductive treatment in 2023-2024 for primary infertility with a history of low fertilization rates (<30%), low blastulation rates (<20%), or male factor infertility such as teratozoospermia at the Izmir University of Economics Medical Point Hospital IVF Center. Sibling oocytes from each patient were randomly assigned to piezoelectric or CaI activation. The fertilization rate, number of degenerated embryos, number of abnormally fertilized eggs, number of day 3 grade 1 embryos, and blastocyst counts were compared among the sibling oocytes. In total, 1,105 MII sibling oocytes from 76 patients were included. Piezoelectric activation was applied to 575 MII oocytes, while CaI activation was applied to 530 MII oocytes. There was no significant difference between groups according to the activation method in the fertilization rate (piezo, 0.74% ± 0.22%; CaI, 0.68% ± 0.26%, = 0.12), degeneration rate (piezo, 0.04% ± 0.13%; CaI, 0.02% ± 0.06%, = 0.452), day 3 grade 1 embryo rate (piezo, 0.59% ± 0.33%; CaI, 0.54% ± 0.35%, = 0.34), or blastocyst rate (piezo, 0.49% ± 0.33%; CaI, 0.43% ± 0.34%, = 0.28) of sibling oocytes. Both piezoelectric and CaI activation yielded comparable results in terms of oocyte activation, with similar fertilization and blastulation rates.
为了确定在接受压电和钙离子载体（CaI）激活的同一患者的同胞卵母细胞中的受精率、优质第3天胚胎率和囊胚发育率。这项回顾性队列研究纳入了2023年至2024年在伊兹密尔经济大学医学点医院体外受精中心因原发性不孕症接受辅助生殖治疗的夫妇，这些夫妇既往受精率低（<30%）、囊胚形成率低（<20%）或存在男性因素不孕症，如畸形精子症。将每位患者的同胞卵母细胞随机分配至压电激活或CaI激活。比较同胞卵母细胞之间的受精率、退化胚胎数量、异常受精卵数量、第3天1级胚胎数量和囊胚数量。总共纳入了来自76名患者的1105个MII期同胞卵母细胞。对575个MII期卵母细胞进行了压电激活，而对530个MII期卵母细胞进行了CaI激活。根据激活方法，同胞卵母细胞的受精率（压电激活组，0.74%±0.22%；CaI激活组，0.68%±0.26%，P = 0.12）、退化率（压电激活组，0.04%±0.13%；CaI激活组，0.02%±0.06%，P = 0.452）、第3天1级胚胎率（压电激活组，0.59%±0.33%；CaI激活组，0.54%±0.35%，P = 0.34）或囊胚率（压电激活组，0.49%±0.33%；CaI激活组，0.43%±0.34%，P = 0.28）在两组之间均无显著差异。压电激活和CaI激活在卵母细胞激活方面产生了类似的结果，受精率和囊胚形成率相似。
