IVI RMA New Jersey, 140 Allen Rd, Basking Ridge NJ, USA.
IVI RMA New Jersey, 140 Allen Rd, Basking Ridge NJ, USA.
Reprod Biomed Online. 2022 Nov;45(5):878-883. doi: 10.1016/j.rbmo.2022.06.021. Epub 2022 Jun 28.
Can 1-day old human unfertilized oocytes activate and blastulate after exposure to calcium ionophore (Ca.I) A23187?
Prospective randomized trial analysis of sibling oocytes. Seventy unfertilized sibling oocytes from 24 couples were randomly split into two groups. In the treatment group, 35 oocytes were cultured with 5-µM Ca.I A23187 for 10 min, washed and cultured until day 6 of development (D+6). The remaining 35 oocytes (control group) were similarly cultured until D+6. Activation, cleavage and blastulation rates were compared between the two groups.
Comparable activation rates were observed in the oocytes incubated with Ca.I A23187 and in the control group (11.4% versus 17.1%; P = 0.49). The cleavage rate observed was 45.7% in both groups. None of the embryos reached blastocyst stage.
Activation and cleavage can occur in unfertilized oocytes after the diagnosis of failure to fertilize. Unfortunately, the prevalence of activation is not affected by exposure to Ca.I A23187. Additionally, these embryos have no tangible reproductive potential as they arrest before reaching the blastocyst stage.
在钙离子载体（Ca.I）A23187 作用下，1 日龄人类未受精卵能否激活并囊胚发育？
对同胞卵的前瞻性随机试验分析。24 对夫妇的 70 个未受精卵随机分为两组。在治疗组中，35 个卵母细胞用 5-µM Ca.I A23187 孵育 10 分钟，洗涤后培养至发育第 6 天（D+6）。其余 35 个卵母细胞（对照组）也在相同条件下培养至 D+6。比较两组的激活、分裂和囊胚发育率。
用 Ca.I A23187 孵育的卵母细胞和对照组的激活率相似（11.4%对 17.1%；P=0.49）。两组的分裂率均为 45.7%。没有胚胎达到囊胚阶段。
在受精失败的诊断后，未受精卵可以发生激活和分裂。不幸的是，暴露于 Ca.I A23187 并不影响激活的普遍性。此外，这些胚胎在到达囊胚阶段之前就停止发育，没有实际的生殖潜力。
