Yang Jing, Shi Hanhan, Cai Lili, Zhang Sensen, Zhao Yuanzeng, Su Xiaojia, Sun Haiyan, Cao Yinping, Li Yongchao
School of Life Sciences, Henan Institute of Science and Technology, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Xinxiang, China.
College of Grain, Oil and Food Science, Yunnan Vocational College of Mechanical and Electrical Technology, Kunming, China.
Front Microbiol. 2025 May 26;16:1595351. doi: 10.3389/fmicb.2025.1595351. eCollection 2025.
In order to study the effects of lactic acid bacteria on the active substances and functions of honeysuckle, metabolomics was used to analyze the differential metabolites of honeysuckle fermented by lactic acid bacteria. The results showed that increased the contents of total phenols, flavonoids and chlorogenic acid by 26.48, 22.59, and 33.57%, respectively, compared with Control Group (CK). The antioxidant capacity and -glucosidase activity of fermented honeysuckle liquid (FLJT) were the strongest, and the scavenging activity and reducing ability of DPPH radical, hydroxyl radical and superoxide anion radical increased by 33.96, 25.60, 58.21 and 39.29%, respectively, compared with unfermented honeysuckle liquid without lactic acid bacteria (LJT). The 991 metabolites with significant differences were screened and enriched into 79 metabolic pathways. The contents of 4-hydroxybenzoic acid, 3, 4-dihydroxyhydrocinnamic acid, chlorogenic acid and 4-hydroxycinnamic acid, which have antioxidant and hypoglycemic activities, and flavonoids in FLJT were increased compared with those in LJT. fermentation could promote the synthesis and accumulation of secondary metabolites in honeysuckle liquid. This study provides a theoretical basis for the development of honeysuckle functional products.
为研究乳酸菌对金银花活性成分及功能的影响，采用代谢组学方法分析乳酸菌发酵金银花的差异代谢产物。结果表明，与对照组（CK）相比，发酵后金银花中总酚、黄酮和绿原酸含量分别提高了26.48%、22.59%和33.57%。发酵金银花液（FLJT）的抗氧化能力和α-葡萄糖苷酶活性最强，与未发酵的无乳酸菌金银花液（LJT）相比，其对DPPH自由基、羟基自由基和超氧阴离子自由基的清除活性及还原能力分别提高了33.96%、25.60%、58.21%和39.29%。筛选出991种差异显著的代谢产物，并富集到79条代谢途径中。与LJT相比，FLJT中具有抗氧化和降血糖活性的4-羟基苯甲酸、3,4-二羟基肉桂酸、绿原酸和4-羟基肉桂酸以及黄酮类化合物的含量均有所增加。发酵可促进金银花液中次生代谢产物的合成与积累。本研究为金银花功能产品的开发提供了理论依据。
