山羊妊娠毒血症不同阶段血清蛋白质组学图谱的变化
Changes in Serum Proteomic Profiles at Different Stages of Pregnancy Toxemia in Goats.
作者信息
Uztimür Murat, Ünal Cennet Nur, Akpınar Gürler
机构信息
Faculty of Veterinary Medicine, Department of Internal Medicine, Bingöl University, Bingol, Türkiye.
Faculty of Medicine, Medical Biology, Kocaeli University, Kocaeli, Türkiye.
出版信息
J Vet Intern Med. 2025 Jul-Aug;39(4):e70139. doi: 10.1111/jvim.70139.
BACKGROUND
In human medicine, serum proteome profiles are used to differentiate metabolic diseases and evaluate their pathophysiology. Understanding the serum proteome profiles of goats with pregnancy toxemia might help identify the proteomes and pathways responsible for the development of this disease and improve diagnosis and treatment.
HYPOTHESIS/AIM: Determine biomarkers that differentiate healthy goats from goats with subclinical pregnancy toxemia (SPT) and clinical pregnancy toxemia (CPT) using serum proteome analysis. In addition, serum protein differences were evaluated to distinguish between SPT and CPT.
ANIMALS
Thirty-five hair goats were included, with 15 in the SPT group, 10 in the CPT group, and 10 in the control group.
METHODS
The cross-sectional study was conducted with animals from goat farms in Bingol, Türkiye, between December 2023 and May 2024. Serum samples were investigated using mass spectrometry-based proteomic analysis.
RESULTS
Proteomic profiles showed significant variation in relative protein abundance. Twenty-five proteins were significantly (p ≤ 0.01) differentially expressed between animals with pregnancy toxemia and those of the control group, with ≥ 2-fold changes in abundance. Proteins involved in cellular, biological, and molecular processes, including processes related to reactive oxygen species, cytokine activation, acute phase response signaling, lipid metabolism, and antimicrobial activity were altered in animals with pregnancy toxemia.
CONCLUSION AND CLINICAL IMPORTANCE
The proteomic candidates identified are biomarkers that may facilitate the diagnosis, treatment, and management of pregnancy toxemia in goats. Serum proteomic biomarkers could be used in rapid tests in the future and may improve the management of pregnancy toxemia on farms.
背景
在人类医学中，血清蛋白质组图谱用于区分代谢性疾病并评估其病理生理学。了解妊娠毒血症山羊的血清蛋白质组图谱可能有助于识别导致该疾病发生的蛋白质组和途径，并改善诊断和治疗。
假设/目的：使用血清蛋白质组分析确定能够区分健康山羊与亚临床妊娠毒血症（SPT）和临床妊娠毒血症（CPT）山羊的生物标志物。此外，评估血清蛋白差异以区分SPT和CPT。
动物
纳入35只毛用山羊，其中SPT组15只，CPT组10只，对照组10只。
方法
2023年12月至2024年5月期间，对来自土耳其宾格尔山羊养殖场的动物进行了横断面研究。使用基于质谱的蛋白质组分析对血清样本进行研究。
结果
蛋白质组图谱显示相对蛋白质丰度存在显著差异。妊娠毒血症动物与对照组动物之间有25种蛋白质显著（p≤0.01）差异表达，丰度变化≥2倍。参与细胞、生物学和分子过程的蛋白质，包括与活性氧、细胞因子激活、急性期反应信号传导、脂质代谢和抗菌活性相关的过程，在妊娠毒血症动物中发生了改变。
结论及临床意义
鉴定出的蛋白质组候选物是可能有助于山羊妊娠毒血症诊断、治疗和管理的生物标志物。血清蛋白质组生物标志物未来可用于快速检测，并可能改善养殖场妊娠毒血症的管理。
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