Du Yuting, Li Xiaojie, Zhang Jiajie, Jie Guifen
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Anal Chem. 2025 Jun 24;97(24):12882-12892. doi: 10.1021/acs.analchem.5c02314. Epub 2025 Jun 10.
In order to solve the problem of low electrochemiluminescence (ECL) intensity and resolution for imaging detection, we innovatively discovered for the first time that the ECL quantum efficiency of the CdS quantum dots (QDs)/g-CN/KSO system is 18.4 times that of the g-CN/KSO system, so CdS QDs/g-CN displays high-intensity ECL and can be used for mobile phone ECL imaging. In particular, CdS QDs combined with g-CN can produce a new color, from the original blue to green, which greatly improves the ECL imaging resolution and detection sensitivity, thus achieving high-resolution visual ECL analysis of the target. Furthermore, CdS QDs/g-CN also shows excellent photoelectrochemical (PEC) properties. Therefore, a unique multimode sensing and colorimetric imaging platform based on CdS QDs/g-CN is constructed. A target was induced to produce many S2 through cyclic amplification. The porous magnetic FeO nanoparticles (NPs) were used to load a large number of CdS QDs, and target-related S2 opened S1 on FeO NPs to release many CdS QD probes, which can further amplify the signal. After convenient magnetic separation for CdS QDs corresponding to the target, the released CdS QDs were added to g-CN nanosheets to realize multimode detection of ECL, imaging, and PEC of . This work not only innovatively found that CdS QDs can change the luminous color of g-CN and CdS QDs/g-CN display high-intensity ECL, but also developed a new PEC-ECL-imaging multimode sensor with excellent performance, which greatly improves the detection sensitivity and accuracy, and makes a significant contribution to the ECL analysis field.
为了解决成像检测中电化学发光（ECL）强度低和分辨率低的问题，我们首次创新性地发现，CdS量子点（QDs）/g-CN/KSO体系的ECL量子效率是g-CN/KSO体系的18.4倍，因此CdS QDs/g-CN表现出高强度的ECL，可用于手机ECL成像。特别是，CdS QDs与g-CN结合可产生新的颜色，从原来的蓝色变为绿色，这大大提高了ECL成像分辨率和检测灵敏度，从而实现了对目标的高分辨率可视化ECL分析。此外，CdS QDs/g-CN还表现出优异的光电化学（PEC）性能。因此，构建了基于CdS QDs/g-CN的独特多模式传感和比色成像平台。通过循环放大诱导靶标产生许多S2。多孔磁性FeO纳米颗粒（NPs）用于负载大量CdS QDs，与靶标相关的S2打开FeO NPs上的S1以释放许多CdS QD探针，这可以进一步放大信号。对与靶标对应的CdS QDs进行便捷的磁分离后，将释放的CdS QDs添加到g-CN纳米片中，以实现对的ECL、成像和PEC的多模式检测。这项工作不仅创新性地发现CdS QDs可以改变g-CN的发光颜色且CdS QDs/g-CN表现出高强度的ECL，还开发了一种性能优异的新型PEC-ECL-成像多模式传感器，大大提高了检测灵敏度和准确性，为ECL分析领域做出了重大贡献。
