Mallett A K, Rowland I R, Bearne C A, Nicklin S
Toxicol Appl Pharmacol. 1985 May;78(3):377-85. doi: 10.1016/0041-008x(85)90243-1.
Rats, mice, and hamsters were fed iota-carrageenan incorporated in a fiber-free, purified diet for 30 days, and the activities of a number of cecal microbial enzymes were determined in vitro. Carrageenan treatment produced cecal enlargement in all species, yet significantly decreased the concentration of bacteria per gram of cecal content. Azoreductase, beta-glucosidase, beta-glucuronidase, nitrate reductase, and nitroreductase activities per gram of cecal content were significantly decreased in the rat, although less consistent effects were found in these enzymes in the mouse and hamster. beta-Glucuronidase and nitrate reductase functions were increased per gram of cecal contents in the hamster. The total activity per cecum of certain of these enzymes was modified by the concomitant cecal enlargement, yet total nitroreductase activity was significantly decreased in all three rodent species. iota-Carrageenan significantly decreased the concentration of enterobacteria, staphylococci, streptococci, lactobacilli, facultative anaerobes, and the total microscopic count in the rat cecum, but did not exert any effect on bacterial viability in vitro. Although having no effect on biliary IgA antibody concentration, iota- and kappa- carrageenan when present at 50 g/kg diet increased the agglutination response of the IgA specific for the hindgut microflora.
将大鼠、小鼠和仓鼠喂食含有iota-卡拉胶的无纤维纯化饮食30天，然后在体外测定一些盲肠微生物酶的活性。卡拉胶处理使所有物种的盲肠均出现肿大，但每克盲肠内容物中的细菌浓度显著降低。每克大鼠盲肠内容物中的偶氮还原酶、β-葡萄糖苷酶、β-葡萄糖醛酸酶、硝酸还原酶和硝基还原酶活性显著降低，不过在小鼠和仓鼠中这些酶的变化不太一致。每克仓鼠盲肠内容物中的β-葡萄糖醛酸酶和硝酸还原酶功能增强。这些酶中某些酶的每个盲肠的总活性因伴随的盲肠肿大而改变，但所有三种啮齿动物的总硝基还原酶活性均显著降低。iota-卡拉胶显著降低大鼠盲肠中肠杆菌、葡萄球菌、链球菌、乳酸杆菌、兼性厌氧菌的浓度以及总显微镜计数，但对体外细菌活力没有任何影响。尽管对胆汁IgA抗体浓度没有影响，但当饮食中含有50 g/kg的iota-卡拉胶和kappa-卡拉胶时，会增加对后肠微生物群特异的IgA的凝集反应。
