Chen Sumin, Cheng Jie, Liu Bingliang, Liu Changying, Li Han, Liu Yanxia, Zou Liang, Li Qiang
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industrialization, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, China.
Guizhou Academy of Tobacco Science, Guiyang, Guizhou, Sichuan 550003, China.
J Hazard Mater. 2025 Sep 5;495:138860. doi: 10.1016/j.jhazmat.2025.138860. Epub 2025 Jun 7.
In this study, a novel bacterial strain, M1, capable of degrading both polystyrene (PS) microplastics and carbendazim, was isolated from soil heavily contaminated with microplastics and fungicide carbendazim, and was identified and determined to be Bacillus velezensis. During the 60-day cultivation period, degradation experiments with single pollutants demonstrated that, at a concentration of 1000 mg/L, the strain achieved mass reduction efficiency of 10.8 ± 0.95 % for PS microplastics and 56.64 ± 0.60 % for carbendazim. Remarkably, under co-exposure conditions with both pollutants (1000 mg/L each), attaining mass reduction efficiency of 6.60 ± 0.85 % for PS microplastics and 34.70 ± 2.52 % for carbendazim respectively. After 60 days of biodegradation, scanning electron microscopy (SEM), water contact angle (WCA), high-temperature gel chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) confirmed the biodegradation of PS microplastics by Bacterium M1 under different conditions. The biodegradation of carbendazim by bacteria under different conditions was confirmed by high performance liquid chromatography (HPLC), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) analyses. This study reports for the first time a novel bacterial strain capable of simultaneous degradation of both PS microplastics and carbendazim, providing a new approach to address composite organic pollution in the environment.
在本研究中，从受微塑料和杀菌剂多菌灵严重污染的土壤中分离出一种新型菌株M1，它能够降解聚苯乙烯（PS）微塑料和多菌灵，并被鉴定为贝莱斯芽孢杆菌。在60天的培养期内，单污染物降解实验表明，在浓度为1000 mg/L时，该菌株对PS微塑料的质量减少效率达到10.8±0.95%，对多菌灵的质量减少效率达到56.64±0.60%。值得注意的是，在两种污染物共同暴露条件下（各1000 mg/L），PS微塑料和多菌灵的质量减少效率分别达到6.60±0.85%和34.70±2.52%。经过60天的生物降解后，扫描电子显微镜（SEM）、水接触角（WCA）、高温凝胶色谱（GPC）、傅里叶变换红外光谱（FTIR）和热重分析（TGA）证实了菌株M1在不同条件下对PS微塑料的生物降解。通过高效液相色谱（HPLC）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）分析证实了细菌在不同条件下对多菌灵的生物降解。本研究首次报道了一种能够同时降解PS微塑料和多菌灵的新型菌株，为解决环境中的复合有机污染提供了一种新方法。
