Xiang Peng, Zhang Yunfeng, Zhang Ting, Wu Qian, Zhao Changsong, Li Qiang
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industrialization, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, China.
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industrialization, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, China; School of Public Health, Chengdu Medical College, Chengdu, Sichuan, China.
J Hazard Mater. 2023 Sep 15;458:131856. doi: 10.1016/j.jhazmat.2023.131856. Epub 2023 Jun 15.
This study aimed to investigate the combined decomposition of polystyrene (PS) microplastics using three bacterial cultures: Stenotrophomonas maltophilia, Bacillus velezensis, and Acinetobacter radioresistens. The ability of all three strains to grow on medium containing PS (Mn 90,000 Da, Mw 241,200 Da) microplastics as the sole carbon source was examined. After 60 days of A. radioresistens treatment, the maximum weight loss of the PS microplastics was found to be 16.7 ± 0.6% (half-life 251.1 d). After 60 days of treatment with S. maltophilia and B. velezensis, the maximum weight loss of PS microplastics was 43.5 ± 0.8% (half-life 74.9 d). After 60 days of treatment with S. maltophilia, B. velezensis, and A. radioresistens, the weight loss of the PS microplastics was 17.0 ± 0.2% (half-life 224.2 d). The S. maltophilia and B. velezensis treatment showed a more significant degradation effect after 60 days. This result was attributed to interspecific assistance and interspecific competition. Biodegradation of PS microplastics was confirmed using scanning electron microscopy, water contact angle, high-temperature gel chromatography, Fourier transform infrared spectroscopy and thermogravimetric analysis. This study is the first to explore the degradation ability of different bacterial combinations on PS microplastics, providing a reference for future research on the biodegradation technology of mixed bacteria.
本研究旨在利用嗜麦芽窄食单胞菌、贝莱斯芽孢杆菌和抗辐射不动杆菌这三种细菌培养物来研究聚苯乙烯（PS）微塑料的联合分解情况。检测了这三种菌株在以PS（Mn 90,000 Da，Mw 241,200 Da）微塑料作为唯一碳源的培养基上生长的能力。经抗辐射不动杆菌处理60天后，发现PS微塑料的最大重量损失为16.7±0.6%（半衰期251.1天）。经嗜麦芽窄食单胞菌和贝莱斯芽孢杆菌处理60天后，PS微塑料的最大重量损失为43.5±0.8%（半衰期74.9天）。经嗜麦芽窄食单胞菌、贝莱斯芽孢杆菌和抗辐射不动杆菌处理60天后，PS微塑料的重量损失为17.0±0.2%（半衰期224.2天）。嗜麦芽窄食单胞菌和贝莱斯芽孢杆菌处理在60天后显示出更显著的降解效果。该结果归因于种间协助和种间竞争。通过扫描电子显微镜、水接触角、高温凝胶色谱、傅里叶变换红外光谱和热重分析证实了PS微塑料的生物降解。本研究首次探索了不同细菌组合对PS微塑料的降解能力，为未来混合细菌生物降解技术的研究提供了参考。
