Tang Yunfei, Xiao Mengmeng, Duo Tongtong, Zhang Xiaowan, Chen Ziyue, Wei Wei
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
ACS Appl Mater Interfaces. 2025 Jun 25;17(25):36478-36486. doi: 10.1021/acsami.5c06476. Epub 2025 Jun 10.
The abnormal expression of monoamine oxidase B (MAO-B) and the soluble oligomer (Aβo) formed by β-amyloid deposition play key roles in the pathogenesis of Alzheimer's disease. However, the simultaneous detection of MAO-B and Aβo remains a huge challenge. Herein, a bimetallic core-shell probe ZIF-8 embedding rhodamine 6G (Au/Rh6G/Pd@ZIF-8) with peroxidase-like activity is designed to detect MAO-B and Aβo simultaneously. MAO-B catalyzes the substrate phenethylamine (PEA) to produce HO, which oxidizes thiamine with the catalysis of Au/Rh6G/Pd@ZIF-8 to produce a fluorescent product that emits a strong fluorescence at 440 nm. When Aβo is present in the system, due to the specific coordination between Aβo and Zn, the intact ZIF-8 structure collapses, leading to Rh6G release and fluorescence recovery. The simultaneous determination of MAO-B and Aβo by Au/Rh6G/Pd@ZIF-8 is realized. The results showed that the designed biosensor had high specificity and sensitivity, a wide detection range, and a low detection limit for MAO-B and Aβo simultaneous detection. Fluorescence signals for MAO-B and Aβo detection did not interfere with each other because they are excited separately, which provides more valuable data for the diagnosis of Alzheimer's disease.
单胺氧化酶B（MAO - B）的异常表达以及由β - 淀粉样蛋白沉积形成的可溶性寡聚体（Aβo）在阿尔茨海默病的发病机制中起关键作用。然而，同时检测MAO - B和Aβo仍然是一个巨大的挑战。在此，设计了一种具有过氧化物酶样活性的双金属核壳探针——包埋罗丹明6G的ZIF - 8（Au/Rh6G/Pd@ZIF - 8），用于同时检测MAO - B和Aβo。MAO - B催化底物苯乙胺（PEA）生成HO，HO在Au/Rh6G/Pd@ZIF - 8的催化下氧化硫胺素，产生在440 nm处发射强荧光的荧光产物。当系统中存在Aβo时，由于Aβo与Zn之间的特异性配位作用，完整的ZIF - 8结构坍塌，导致罗丹明6G释放并使荧光恢复。实现了用Au/Rh6G/Pd@ZIF - 8同时测定MAO - B和Aβo。结果表明，所设计的生物传感器具有高特异性和灵敏度、宽检测范围以及对MAO - B和Aβo同时检测的低检测限。用于检测MAO - B和Aβo的荧光信号相互不干扰，因为它们是分别激发的，这为阿尔茨海默病的诊断提供了更有价值的数据。
