Kaufman G E
Ultrasound Med Biol. 1985 May-Jun;11(3):497-501. doi: 10.1016/0301-5629(85)90162-0.
Chinese hamster V79 cells were exposed in suspension to 1 MHz continuous wave ultrasound at spatial peak intensities of 7 and 35 W/cm2. Exposure durations were from 10 to 180 sec, so as to encompass the first decade of decreased survival at each intensity. Exposure at 35 W/cm2 resulted in the induction of 6-thioguanine-resistant mutants. The mutation frequency was approximately one-tenth that induced by x rays at similar survival levels. No mutagenesis was observed at 7 W/cm2. Results are consistent with free radical formation as the mechanism responsible for ultrasound-induced mutagenesis.
将中国仓鼠V79细胞悬浮培养，暴露于空间峰值强度分别为7和35 W/cm²的1 MHz连续波超声中。暴露持续时间为10至180秒，以涵盖每个强度下存活率下降的第一个十年。35 W/cm²的暴露导致了6-硫鸟嘌呤抗性突变体的诱导。突变频率约为在相似存活水平下X射线诱导频率的十分之一。在7 W/cm²时未观察到诱变作用。结果与自由基形成作为超声诱导诱变的机制一致。
