Badawy Sherouk S, Mashaly Mohammad M, Abdel-Aziz A F, Madkour Mai M
Department of Chemistry, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
Department of Chemistry, Faculty of Science, Damietta University, New Damietta 34517, Egypt.
Toxicol Res (Camb). 2025 Jun 9;14(3):tfaf076. doi: 10.1093/toxres/tfaf076. eCollection 2025 Jun.
The main objective of this research was to investigate the therapeutic, anti-inflammatory, and histological effects of metformin and taurine, both alone and in combination, against thioacetamide (TAA)-induced hepatotoxicity in rats. The study included forty adult male Swiss albino rats, which were divided into five groups: Group I provided the control group. Group II (TAA group) rats received injections of TAA (200 mg/kg b.wt /3 times/week, i.p.) for six weeks. Group III (TAA + metformin) rats received administration of metformin (200 mg/kg/day, p.o.) for five weeks. Group IV (TAA + taurine) rats received injections of taurine (100 mg/kg/day, i.p.) for five weeks, while Group V (TAA + metformin + taurine) rats received daily intraperitoneal injections and oral administration of the medication for five weeks. Inflammation and changes in liver function are hallmarks of TAA-induced hepatotoxicity. Our findings demonstrated that the greatly improved liver dysfunction might be attributed to the effects of metformin and taurine. Furthermore, a combination of metformin and taurine markedly inhibited inflammatory responses, as indicated by the decreased levels of the inflammatory cytokine IL-6. The biochemical results were confirmed by the histological analyses of the liver tissues. Post-treatments of metformin and taurine might have crucial potential and synergistic effects against TAA-induced hepatotoxicity.
本研究的主要目的是研究二甲双胍和牛磺酸单独及联合使用对硫代乙酰胺（TAA）诱导的大鼠肝毒性的治疗、抗炎和组织学作用。该研究包括40只成年雄性瑞士白化大鼠，分为五组：第一组为对照组。第二组（TAA组）大鼠接受TAA注射（200mg/kg体重/每周3次，腹腔注射），持续六周。第三组（TAA + 二甲双胍）大鼠接受二甲双胍给药（200mg/kg/天，口服），持续五周。第四组（TAA + 牛磺酸）大鼠接受牛磺酸注射（100mg/kg/天，腹腔注射），持续五周，而第五组（TAA + 二甲双胍 + 牛磺酸）大鼠接受每日腹腔注射和口服药物，持续五周。炎症和肝功能变化是TAA诱导的肝毒性的标志。我们的研究结果表明，肝功能障碍的显著改善可能归因于二甲双胍和牛磺酸的作用。此外，二甲双胍和牛磺酸的组合显著抑制了炎症反应，炎症细胞因子IL-6水平降低表明了这一点。肝脏组织的组织学分析证实了生化结果。二甲双胍和牛磺酸的治疗后可能对TAA诱导的肝毒性具有关键的潜在协同作用。
