Chang Runlei, Yan Zhaoqi, Jiang Jibo, Wang Yichen, Si Hongli, Bose Tanay, Miao Congcong
College of Life Science, Shandong Normal University, Jinan, China Shandong Normal University Jinan China.
Dongying Institute, Shandong Normal University, Dongying, China Shandong Normal University Dongying China.
MycoKeys. 2025 Jun 2;118:55-80. doi: 10.3897/mycokeys.118.155248. eCollection 2025.
is one of the largest and most diverse genera of fruticose lichens with global distribution. Endolichenic fungi, which thrive within lichen thalli, have emerged as a promising source of bioactive compounds, with the ability to synthesise a variety of metabolites with biopharmaceutical potential. In this study, four isolates of endolichenic fungi isolated from spp. were identified using comprehensive multi-gene phylogenetic analyses. These isolates were evaluated for their anticancer, antifungal, and antibacterial properties, as well as for their ability to produce extracellular enzymes. Our findings revealed that the isolates represent four novel species, named as , , , and . Our screening assay showed and exhibited cytotoxic effects against the H460 human lung cancer cell line, with moderate inhibitory activity at a concentration of 100 μg/mL. The four fungal isolates exhibited distinct antifungal profiles against phytopathogens: specifically inhibited , while showed broad-spectrum activity against , , and . No antibacterial effects were detected in any isolates. These fungi exhibited a diverse array of extracellular enzyme activities, including amylase, protease, gelatinase, glucose oxidase, and cellulase. Collectively, these results underscore the considerable biotechnological potential of endolichenic fungi as sources of bioactive compounds with applications in drug discovery, agriculture, and environmental management. These findings also highlight the ecological importance of endolichenic fungi, suggesting that they may play multifaceted roles in lichen symbioses and their environments. Continued exploration of these fungi is essential for unlocking their full pharmacological and industrial potential.
是枝状地衣中最大且最多样化的属之一，分布于全球。内生地衣真菌在地衣菌体中蓬勃生长，已成为有前景的生物活性化合物来源，能够合成多种具有生物制药潜力的代谢物。在本研究中，使用全面的多基因系统发育分析鉴定了从 物种分离出的四种内生地衣真菌分离株。对这些分离株进行了抗癌、抗真菌和抗菌特性评估，以及产生胞外酶能力的评估。我们的研究结果表明，这些分离株代表四个新物种，命名为 、 、 和 。我们的筛选试验表明， 和 对H460人肺癌细胞系表现出细胞毒性作用，在浓度为100μg/mL时具有中等抑制活性。这四种真菌分离株对植物病原体表现出不同的抗真菌谱： 特异性抑制 ，而 对 、 和 表现出广谱活性。在任何分离株中均未检测到抗菌作用。这些真菌表现出多种胞外酶活性，包括淀粉酶、蛋白酶、明胶酶、葡萄糖氧化酶和纤维素酶。总体而言，这些结果强调了内生地衣真菌作为生物活性化合物来源在药物发现、农业和环境管理中的应用具有相当大的生物技术潜力。这些发现还突出了内生地衣真菌的生态重要性，表明它们可能在地衣共生及其环境中发挥多方面作用。持续探索这些真菌对于释放其全部药理和工业潜力至关重要。
