Besançon Hervé, Polidori Margherita, Hostettler Andrea, Nizet Victor, Oevermann Anna, Babiychuk Eduard
Institute of Anatomy, University of Bern, Bern, Switzerland.
Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California, San Diego, La Jolla, California, USA.
Infect Microbes Dis. 2025 Apr 17. doi: 10.1097/IM9.0000000000000177.
is a human and veterinary pathogen, one of the most common agents of foodborne infections worldwide. It can cause severe complications such as meningitis or miscarriage. Anti-virulence therapies, which target virulence factors such as pore-forming toxins, offer an alternative approach to combating infections. In this study, cholesterol-containing liposomal nanotraps effectively neutralized exotoxins, particularly listeriolysin O (LLO), thereby protecting mammalian cells. Notably, toxin neutralization was observed under both neutral and acidic conditions, where LLO activity is optimized to facilitate bacterial escape from the phagosome. Liposomal nanotraps were phagocytosed by macrophages and colocalized with intracellular , increasing the clearance rate of intracellular bacteria. These findings expand the potential use of broad-spectrum liposomal nanotrap therapy, which could be employed alongside current standard of care treatments to assist the immune system in controlling virulent pathogens.
它是一种人类和兽医病原体，是全球食源性感染最常见的病原体之一。它可导致严重并发症，如脑膜炎或流产。针对诸如成孔毒素等毒力因子的抗毒力疗法提供了一种对抗感染的替代方法。在本研究中，含胆固醇的脂质体纳米陷阱有效地中和了外毒素，特别是溶血素O（LLO），从而保护了哺乳动物细胞。值得注意的是，在中性和酸性条件下均观察到毒素中和，在酸性条件下LLO活性被优化以促进细菌从吞噬体中逃逸。脂质体纳米陷阱被巨噬细胞吞噬并与细胞内的（此处原文似乎不完整）共定位，提高了细胞内细菌的清除率。这些发现扩展了广谱脂质体纳米陷阱疗法的潜在用途，该疗法可与当前的标准护理治疗一起使用，以协助免疫系统控制有毒病原体。
