Voros Charalampos, Varthaliti Antonia, Athanasiou Diamantis, Mavrogianni Despoina, Papahliou Anthi-Maria, Bananis Kyriakos, Koulakmanidis Aristotelis-Marios, Athanasiou Antonia, Athanasiou Aikaterini, Zografos Constantinos G, Gkirgkinoudis Athanasios, Daskalaki Maria Anastasia, Mazis Kourakos Dimitris, Vaitsis Dimitrios, Papapanagiotou Ioannis, Theodora Marianna, Antsaklis Panagiotis, Loutradis Dimitrios, Daskalakis Georgios
1st Department of Obstetrics and Gynecology, 'Alexandra' General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece.
King's College Hospitals NHS Foundation Trust, London SE5 9RS, UK.
Cells. 2025 May 27;14(11):787. doi: 10.3390/cells14110787.
MicroRNAs (miRNAs) in follicular fluid (FF) are being recognized as important regulators of ovarian function and biomarkers of reproductive success. This systematic analysis investigates FF-derived miRNAs and their relationship to polycystic ovarian syndrome (PCOS) and in vitro fertilization (IVF) outcomes. Following PRISMA recommendations, 21 original papers were included that looked at miRNA expression in FF or granulosa cells from women undergoing IVF, with or without PCOS. The study design, miRNA profiling methodologies, IVF protocols, and clinical results were gathered and analyzed. Across the investigations, 15 miRNAs were regularly implicated, including miR-132, miR-320, miR-222, miR-224, miR-146a, and miR-93. Downregulation of miR-132 and miR-320 was consistently detected in PCOS and associated with decreased steroidogenesis. Elevated miR-222 and miR-146a were linked to insulin resistance and follicular inflammation. In IVF, miR-202-5p and miR-224 were elevated in high-quality embryos and successful cycles, indicating that they have roles in granulosa cell proliferation and estrogen synthesis. MiRNA dysregulation was linked to critical pathways, such as PI3K/AKT, NF-κB, TGF-β, and WNT. Specific FF miRNAs are consistently linked to PCOS pathogenesis and IVF effectiveness. Their use into noninvasive biomarker panels could improve embryonic selection and personalized reproductive care.
卵泡液（FF）中的微小RNA（miRNA）正被视为卵巢功能的重要调节因子和生殖成功的生物标志物。这项系统分析研究了源自卵泡液的miRNA及其与多囊卵巢综合征（PCOS）和体外受精（IVF）结果的关系。按照PRISMA指南，纳入了21篇原创论文，这些论文研究了接受IVF的女性（无论是否患有PCOS）卵泡液或颗粒细胞中的miRNA表达。收集并分析了研究设计、miRNA谱分析方法、IVF方案和临床结果。在各项研究中，15种miRNA经常被涉及，包括miR-132、miR-320、miR-222、miR-224、miR-146a和miR-93。在PCOS中持续检测到miR-132和miR-320的下调，且与类固醇生成减少有关。miR-222和miR-146a的升高与胰岛素抵抗和卵泡炎症有关。在IVF中，高质量胚胎和成功周期中miR-202-5p和miR-224升高，表明它们在颗粒细胞增殖和雌激素合成中起作用。miRNA失调与关键信号通路有关，如PI3K/AKT、NF-κB、TGF-β和WNT。特定的卵泡液miRNA与PCOS发病机制和IVF有效性始终相关。将它们用于无创生物标志物组合可以改善胚胎选择和个性化生殖护理。
