Wang Xi'ang, Zhu Bin, Winn Robert, Lu Shanfa, Wang Hengbin
Department of Internal Medicine, Division of Hematology, Oncology and Palliative Care, Massey Comprehensive Cancer Center, School of Medicine, Virginia Commonwealth University, Richmond, VI 23298, USA.
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Cells. 2025 May 29;14(11):796. doi: 10.3390/cells14110796.
Arginine methylation is a crucial post-translational modification (PTM) that plays a significant role in various biological processes. It occurs in two primary forms: mono-methylation (MMA) and di-methylation (DMA), with the latter further classified into symmetric (SDMA) and asymmetric methylation (ADMA). This review examines the functional implications of these methylation states, current detection methodologies, proteomics-based analytical approaches, and the different impacts of these methylations on protein function. Finally, the role of protein arginine methyltransferases (PRMTs) and their substrate specificity in shaping the arginine methylome are discussed.
精氨酸甲基化是一种关键的翻译后修饰（PTM），在各种生物学过程中发挥着重要作用。它以两种主要形式出现：单甲基化（MMA）和二甲基化（DMA），后者进一步分为对称二甲基化（SDMA）和不对称甲基化（ADMA）。本综述探讨了这些甲基化状态的功能意义、当前的检测方法、基于蛋白质组学的分析方法，以及这些甲基化对蛋白质功能的不同影响。最后，讨论了蛋白质精氨酸甲基转移酶（PRMTs）的作用及其底物特异性在塑造精氨酸甲基化组中的作用。
