Egyed-Kolumbán Abigél, Onhausz Benita, Barta Bence Pál, Szalai Zita, Huliák Ildikó, Kiricsi Mónika, Bagyánszki Mária, Bódi Nikolett
Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary.
Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary.
Cells. 2025 May 30;14(11):809. doi: 10.3390/cells14110809.
Enteric neurons regulating motility display regional damage to diabetes. By inhibiting neuroinflammation, insulin can contribute to neuronal survival, therefore, we aimed to investigate the presence of insulin in myenteric neurons and their nitrergic population in acute and chronic rat models of type 1 diabetes. One or ten weeks after the onset of hyperglycemia, gut segments and the pancreas of control, diabetic, and insulin-treated diabetic rats were investigated. In the controls, insulin-immunoreactive neurons comprised 8-9% of the total myenteric neuronal population in the ileum and colon and 2-4% in the duodenum. Except for the duodenum, this proportion was significantly increased in acute hyperglycemic rats and was decreased in the colon of the chronic ones. However, the proportion of insulin-immunoreactive nitrergic neurons remained unchanged in all segments in chronic hyperglycemia. Immunogold electron microscopy revealed an increased density of insulin-labelling gold particles in diabetic duodenal ganglia of the chronic experiment. Insulin mRNA was not detected in intestinal samples either in controls or diabetics. These findings support time-dependent and regional alterations in the proportion of insulin-immunoreactive myenteric neurons and their nitrergic subpopulation. Regionally different insulin content of myenteric neurons may contribute to their protection from diabetic damage.
调节运动的肠神经元在糖尿病中表现出局部损伤。通过抑制神经炎症，胰岛素可促进神经元存活，因此，我们旨在研究1型糖尿病急性和慢性大鼠模型中肌间神经丛神经元及其含氮能神经元群体中胰岛素的存在情况。在高血糖发作1周或10周后，对对照、糖尿病和胰岛素治疗的糖尿病大鼠的肠道段和胰腺进行了研究。在对照组中，胰岛素免疫反应性神经元在回肠和结肠中占肌间神经丛神经元总数的8-9%，在十二指肠中占2-4%。除十二指肠外，急性高血糖大鼠的这一比例显著增加，而慢性高血糖大鼠结肠中的这一比例则降低。然而，在慢性高血糖状态下，所有肠段中胰岛素免疫反应性含氮能神经元的比例保持不变。免疫金电子显微镜显示，在慢性实验的糖尿病十二指肠神经节中，胰岛素标记金颗粒的密度增加。在对照组或糖尿病组的肠道样本中均未检测到胰岛素mRNA。这些发现支持了胰岛素免疫反应性肌间神经丛神经元及其含氮能亚群比例的时间依赖性和区域改变。肌间神经丛神经元区域不同的胰岛素含量可能有助于其免受糖尿病损伤。
