Cnb蛋白在异源基因表达系统中影响细胞内2-甲基异莰醇的保留。

Cnb proteins affect the intracellular 2-MIB retention in heterologous gene expression systems.

作者信息

Dayarathne Kaushalya, Atsuzawa Kimie, Ishikawa Toshiki, Watanabe Satoru, Kaneko Yasuko, Kadeer Aikeranmu, Yamaguchi Masatoshi, Kawai-Yamada Maki

机构信息

Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.

Comprehensive Analysis Center for Science, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama-city, 338-8570, Saitama, Japan.

出版信息

Arch Microbiol. 2025 Jun 11;207(8):172. doi: 10.1007/s00203-025-04377-1.

DOI:10.1007/s00203-025-04377-1

PMID:40498159

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12159092/

Abstract

UNLABELLED

2-methylisoborneol (2-MIB) is one of the main cyanobacterial metabolites responsible for unpleasant taste and odor in drinking water. 2-MIB biosynthesis involves a two-step enzymatic reaction catalyzed by enzymes encoded by the and genes. Two homologous genes, A and B, which encode cyclic nucleotide-binding proteins of unknown function are located upstream and downstream of the and genes, respectively. This study investigated the involvement of genes of 2-MIB-producing cyanobacterium var. NIES 512 in 2-MIB synthesis using heterologous gene expression systems. Transgenic and PCC 7942 strains expressing the AB gene cluster exhibited a notable intracellular 2-MIB retention which was not shown by the transgenic strains expressing only the - gene unit. Immunoelectron microscopy detected that the Cnb proteins are associated with unknown intracellular compartments in transgenic . PCC 7942 cells. This study suggests that 2-MIB-related Cnb proteins are involved in the formation of intracellular compartments, which are likely associated with intracellular 2-MIB retention.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s00203-025-04377-1.

摘要

未标记

2-甲基异冰片（2-MIB）是导致饮用水产生难闻气味的主要蓝藻代谢产物之一。2-MIB的生物合成涉及由和基因编码的酶催化的两步酶促反应。两个同源基因A和B分别位于和基因的上游和下游，它们编码功能未知的环核苷酸结合蛋白。本研究使用异源基因表达系统研究了产2-MIB的蓝藻变种NIES 512的基因在2-MIB合成中的作用。表达AB基因簇的转基因和PCC 7942菌株表现出显著的细胞内2-MIB保留，而仅表达 -基因单元的转基因菌株则未表现出这种保留。免疫电子显微镜检测到Cnb蛋白与转基因PCC 7942细胞中未知的细胞内区室相关。本研究表明，与2-MIB相关的Cnb蛋白参与细胞内区室的形成，这可能与细胞内2-MIB的保留有关。