Artati Anna, Couacault Pauline, Witting Michael
Metabolomics and Proteomics Core, Helmholtz Zentrum München GmbH, Neuherberg, Germany.
Chair of Analytical Food Chemistry, TUM School of Life Sciences, Technical University of Munich, Freising, Germany.
Methods Mol Biol. 2025;2925:1-23. doi: 10.1007/978-1-0716-4534-5_1.
Nontargeted metabolomics aims to capture as many metabolites as possible present in a biological system under a certain condition. Despite several analytical techniques commonly applied for nontargeted metabolomics, liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HRMS/MS) is one of the most employed techniques. To cover broad metabolic classes, different chromatographic separation techniques are implemented, i.e., reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) techniques. Each method offers a complementary polarity range. While RP covers semi- to nonpolar metabolites, such as fatty acids, acylcarnitines, steroids, bile acids, etc., HILIC allows the analysis of polar metabolites, such as amino acids, amines, and organic acids.Nontargeted metabolomics platforms at Helmholtz Munich have been validated for various biological matrices, including biological liquids (plasma, serum, urine), tissues (liver, heart), and cell samples of human and rodent. Analysis is based on the Sciex ZenoTOF 7600 and three chromatographic separation methods, which can be used individually or in combination.
非靶向代谢组学旨在捕获特定条件下生物系统中尽可能多的代谢物。尽管有几种常用于非靶向代谢组学的分析技术，但液相色谱联用高分辨率串联质谱（LC-HRMS/MS）是最常用的技术之一。为了涵盖广泛的代谢类别，采用了不同的色谱分离技术，即反相（RP）和亲水作用液相色谱（HILIC）技术。每种方法都提供了互补的极性范围。RP可覆盖半极性至非极性代谢物，如脂肪酸、酰基肉碱、类固醇、胆汁酸等，而HILIC则可分析极性代谢物，如氨基酸、胺和有机酸。慕尼黑亥姆霍兹中心的非靶向代谢组学平台已针对各种生物基质进行了验证，包括生物液体（血浆、血清、尿液）、组织（肝脏、心脏）以及人和啮齿动物的细胞样本。分析基于Sciex ZenoTOF 7600和三种色谱分离方法，这些方法可以单独使用或组合使用。
