LC-MS-Based Metabolic Fingerprinting of Vitreous Humor.

Vitreous humor (VH) is the largest structure in the eye that fills the space between the lens and the retina. Its composition and level of metabolites may reflect the physiological and pathophysiological processes occurring in the retina. A protocol for the preparation of VH samples for untargeted metabolomics was developed, and extracted metabolites were analyzed using liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-QTOF-MS). Two types of chromatography were combined with mass spectrometry, reversed-phase (RP-LC-MS), and hydrophilic interaction (HILIC-LC-MS).