Boufeker Sana, Bahi Ahlem, Torche Imene, Boudersa Yacer, Shaikh-Ibrahim Ali, Bousaoula Monna Taki Ellh, Hejazi Sondos
Laboratory of Microbiological Engineering and Applications, University of Mentouri Brothers Constantine 1, Constantine, Algeria.
Laboratory of Genetic, Biochemistry and Plants Biotechnology, University of Mentouri Brothers Constantine 1, Constantine, Algeria.
Protein J. 2025 Jun 11. doi: 10.1007/s10930-025-10277-6.
Plant-derived lectins are getting growing interest for their potential biomedical applications, however, there are few studies on lectins from Eucalyptus vicina fruits. The aim of the current study was to isolate and characterize a new phytolectin from Eucalyptus vicina fruits (EVL) and evaluate its immunomodulatory activity. EVL was purified through ammonium sulfate precipitation (70%) followed by ion-exchange chromatography. The purified protein appeared to have a molecular weight of around 55 kDa, determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE. EVL had strong hemagglutination activity against rabbit erythrocytes and all human blood types and displayed a high affinity against fetuin. The lectin exhibited optimal activity at alkaline pH (8-10) and was remarkably thermostable for 1 h at 70 degrees. Metal ions did not alter activity to a significant degree. The immunomodulatory effects were assessed in vivo using a carbon clearance assay, and EVL injection resulted in a dose-dependent increase in phagocytic activity. Overall, our data suggests that EVL could be a valuable candidate for research and future immunomodulatory therapies.
植物源凝集素因其潜在的生物医学应用而越来越受到关注，然而，关于维氏桉果实凝集素的研究却很少。本研究的目的是从维氏桉果实中分离和鉴定一种新的植物凝集素（EVL），并评估其免疫调节活性。通过硫酸铵沉淀（70%），然后进行离子交换色谱法纯化EVL。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定，纯化后的蛋白质分子量约为55 kDa。EVL对兔红细胞和所有人类血型具有很强的血凝活性，并且对胎球蛋白表现出高亲和力。该凝集素在碱性pH值（8-10）下表现出最佳活性，在70℃下1小时具有显著的热稳定性。金属离子对活性没有显著影响。使用碳清除试验在体内评估免疫调节作用，注射EVL导致吞噬活性呈剂量依赖性增加。总体而言，我们的数据表明，EVL可能是研究和未来免疫调节治疗的有价值候选物。
