Del Monte M, Citti L, Gervasi P G
Xenobiotica. 1985 Jul;15(7):591-7. doi: 10.3109/00498258509045888.
Mouse-liver microsomal mono-oxygenases metabolize isoprene to the corresponding mono-epoxides. The reaction was NADPH- and O2-dependent and was inhibited by CO, SKF525-A and metyrapone. 3,4-Epoxy-3-methyl-1-butene was the major metabolite of isoprene, and the kinetic constants (Km and Vmax) for this epoxidation were determined by analysing the corresponding diol by g.l.c. in incubations with microsomes from control or pretreated mice. 3,4-Epoxy-2-methyl-1-butene was a minor metabolite (approx. 20%). 3,4-Epoxy-2-methyl-1-butene was epoxidated further to the mutagenic isoprene dioxide by microsomes from control or pretreated mice. The Km and Vmax were determined and phenobarbital shown to be an inducer of this epoxidation.
小鼠肝脏微粒体单加氧酶将异戊二烯代谢为相应的单环氧化物。该反应依赖于NADPH和O₂，并受到CO、SKF525 - A和甲吡酮的抑制。3,4 - 环氧 - 3 - 甲基 - 1 - 丁烯是异戊二烯的主要代谢产物，通过气相色谱分析相应的二醇来测定该环氧化反应的动力学常数（Km和Vmax），实验使用来自对照或预处理小鼠的微粒体进行孵育。3,4 - 环氧 - 2 - 甲基 - 1 - 丁烯是次要代谢产物（约20%）。来自对照或预处理小鼠的微粒体可将3,4 - 环氧 - 2 - 甲基 - 1 - 丁烯进一步环氧化为致突变的二氧化异戊二烯。测定了Km和Vmax，并表明苯巴比妥是该环氧化反应的诱导剂。
