异戊二烯在各种啮齿动物体内的肝微粒体代谢

Hepatic microsomal metabolism of isoprene in various rodents.

作者信息

Longo V, Citti L, Gervasi P G

出版信息

Toxicol Lett. 1985 Dec;29(1):33-7. doi: 10.1016/0378-4274(85)90196-1.

Abstract

Microsomal monooxygenases of various rodents metabolise isoprene to the corresponding monoepoxides, 3,4-epoxy-3-methyl-1-butene and 3,4-epoxy-2-methyl-1-butene. The kinetic constants (Km and Vmax) for the formation of the major products were determined by gas-liquid chromatography (GLC). The minor product was further epoxidised to the mutagenic isoprene dioxide by the microsomes of all rodents studied. The Km and Vmax for this subsequent epoxidation were determined and phenobarbital was found to be a good inducer in all species.

摘要

各种啮齿动物的微粒体单加氧酶将异戊二烯代谢为相应的单环氧化物，即3,4-环氧-3-甲基-1-丁烯和3,4-环氧-2-甲基-1-丁烯。通过气液色谱法（GLC）测定了主要产物形成的动力学常数（Km和Vmax）。在所研究的所有啮齿动物的微粒体中，次要产物进一步环氧化为致突变性的二氧化异戊二烯。测定了这种后续环氧化反应的Km和Vmax，发现苯巴比妥在所有物种中都是一种良好的诱导剂。