Ge Jia, Sun Xiaoyu, Li Xinxin, Yang Yu, Zhang Lin, Qu Lingbo, Li Zhaohui
College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China; School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China.
Food Chem. 2025 Oct 30;490:145066. doi: 10.1016/j.foodchem.2025.145066. Epub 2025 Jun 6.
Herein, a novel colorimetric strategy based on Cu-CN single-atom nanozymes (Cu-CN SANs) is constructed for acrylamide detection. Cu-CN SANs exhibit excellent oxidase mimetic activity, which can efficiently catalyze the oxidation of substrate TMB to produce a blue product. GSH inhibits the oxidation of TMB by Cu-CN SANs. However, the addition of acrylamide enables the efficient thiolene Michael addition reaction between GSH and acrylamide, thus restoring the oxidation of TMB by Cu-CN SANs. The developed method enables acrylamide detection within a linear range of 0.05-15 μM, with a low detection limit (LOD) of 15.4 nM and a relative standard deviation (RSD%) below 4.3 %. When applied to real samples, the biosensor achieved satisfactory recoveries of 95.3-103.6 % for acrylamide analysis in bread, cookies, and chips samples. Additionally, integrating the sensor with a smartphone enabled on-site detection, yielding an LOD of 146 nM.
在此，构建了一种基于铜-氰基单原子纳米酶（Cu-CN SANs）的新型比色策略用于丙烯酰胺检测。Cu-CN SANs表现出优异的氧化酶模拟活性，能够高效催化底物3,3',5,5'-四甲基联苯胺（TMB）氧化生成蓝色产物。谷胱甘肽（GSH）抑制Cu-CN SANs对TMB的氧化。然而，加入丙烯酰胺可使GSH与丙烯酰胺之间高效发生硫醇-烯迈克尔加成反应，从而恢复Cu-CN SANs对TMB的氧化。所开发的方法能够在0.05 - 15 μM的线性范围内检测丙烯酰胺，检测限低至15.4 nM，相对标准偏差（RSD%）低于4.3%。当应用于实际样品时，该生物传感器对面包、饼干和薯片样品中丙烯酰胺分析的回收率达到95.3 - 103.6%，令人满意。此外，将该传感器与智能手机集成可实现现场检测，检测限为146 nM。
