Shi Ruirui, Wang Chao, Zhu Mingyue, Xie Chen, Cui Wenyu, Zhou Qing
College of Life Science and Engineering, Jining University, Jining, 273199, China.
College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, China.
Mikrochim Acta. 2025 Jun 11;192(7):414. doi: 10.1007/s00604-025-07277-9.
Manganese dioxide nanozymes (MnO-zymes) with excellent oxidase-like properties were synthesized via a one-step solvothermal method. A rapid, portable smartphone-assisted colorimetric paper sensor based on MnO-zymes/TMB chromogenic system was developed for total antioxidant capacity (TAC) evaluation. The MnO-zymes immediately catalyzed the oxidation of colorless TMB to generate blue oxidation products (oxTMB), while antioxidants can quickly reduce oxTMB to TMB, causing noticeable fading of the blue color. Integrated with a smartphone-based RGB mode, ascorbic acid (AA) was quantified in the range 2.5-125.00 μM with a limit of detection (LOD) of 3.03 μM. Furthermore, using AA as a model analyte, the paper sensor was successfully applied to evaluate TAC in fruits and beverages, with results closely matching those obtained from a microplate reader-assisted colorimetric sensor and an ABTS free radical scavenging capacity assay kit. This high-performance sensor is simple, rapid, and accurate, showing great potential in practical application for TAC determination.
通过一步溶剂热法合成了具有优异类氧化酶性质的二氧化锰纳米酶（MnO-zymes）。基于MnO-zymes/TMB显色体系，开发了一种快速、便携式的智能手机辅助比色纸传感器，用于总抗氧化能力（TAC）评估。MnO-zymes能立即催化无色的TMB氧化生成蓝色氧化产物（oxTMB），而抗氧化剂可迅速将oxTMB还原为TMB，导致蓝色显著褪色。结合基于智能手机的RGB模式，抗坏血酸（AA）的定量范围为2.5 - 125.00 μM，检测限（LOD）为3.03 μM。此外，以AA作为模型分析物，该纸传感器成功应用于水果和饮料中TAC的评估，结果与酶标仪辅助比色传感器和ABTS自由基清除能力检测试剂盒所得结果密切匹配。这种高性能传感器简单、快速且准确，在TAC测定的实际应用中显示出巨大潜力。
