Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam.
Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam.
Parasitology. 2022 Nov;149(13):1709-1719. doi: 10.1017/S0031182022001184. Epub 2022 Aug 23.
The complete circular mitogenome of (Platyhelminthes: Paragonimidae) from Japan, obtained by PacBio long-read sequencing, was 17 591 bp and contained 12 protein-coding genes (PCGs), 2 mitoribosomal RNA and 22 transfer RNA genes. The 8 gene was absent, and there was a 40 bp overlap between 4L and 4. The long non-coding region (4.3 kb) included distinct types of long and short repeat units. The pattern of base usage for PCGs and the mtDNA coding region overall in Asian and American species (, , and ) and the Indian form of was > > > . On the other hand, East-Asian used > > > . Five Asian and American species and had TTT/Phe, TTG/Leu and GTT/Val as the most frequently used codons, whereas the least-used codons were different in each species and between regional forms of . The phylogenetic tree reconstructed from a concatenated alignment of amino acids of 12 PCGs from 36 strains/26 species/5 families of trematodes confirmed that the Paragonimidae is monophyletic, with 100% nodal support. was resolved as a sister to The clade was clearly separate from remaining congeners. The latter clade was comprised of 2 subclades, one of the East-Asian and the other of the Indian Type 1 samples. Additional mitogenomes in the Paragonimidae are needed for genomic characterization and are useful for diagnostics, identification and genetic/ phylogenetic/ epidemiological/ evolutionary studies of the Paragonimidae.
日本圆形并殖吸虫（Platyhelminthes: Paragonimidae）的完整圆形线粒体基因组，通过 PacBio 长读测序获得，大小为 17591bp，包含 12 个蛋白编码基因（PCGs）、2 个线粒体核糖体 RNA 和 22 个转移 RNA 基因。缺失 8 基因，4L 和 4 之间有 40bp 的重叠。长非编码区（4.3kb）包括不同类型的长和短重复单元。亚洲和美洲的 种（、、和）以及印度型 的 PCGs 和整个 mtDNA 编码区的碱基使用模式为 > > > > 。另一方面，东亚的 则为 > > > > 。亚洲和美洲的 5 个种以及 具有 TTT/Phe、TTG/Leu 和 GTT/Val 作为最常用的密码子，而每种物种和 的区域形式之间最少使用的密码子都不同。从 36 株/26 种/5 科吸虫的 12 个 PCGs 氨基酸串联排列重建的系统发育树证实，并殖科是单系的，具有 100%的节点支持。被解析为 姐妹群。 支系明显与其余近缘种分开。后一个分支由东亚和印度 1 型的两个亚分支组成。并殖科需要更多的线粒体基因组进行基因组特征分析，这对于并殖科的诊断、鉴定以及遗传/系统发育/流行病学/进化研究都是有用的。
