Ma Chi, Yang Juan, Fu Xin-Nian, Luo Jiang-Yan, Liu Pei, Zeng Xue-Li, Li Xin-Yi, Zhang Shun-Ling, Zheng Sheng
Department of Gastroenterology, The Second Affiliated Hospital of Dali University, Kunming 650011, Yunnan Province, China.
Department of Gastroenterology, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan Province, China.
World J Hepatol. 2025 May 27;17(5):106124. doi: 10.4254/wjh.v17.i5.106124.
In this study, we are committed to exploring the characteristics of the gut microbiome in three different stages of chronic liver disease (CLD): Chronic hepatitis B, liver cirrhosis, and hepatocellular carcinoma (HCC).
To delineate the gut microbiota traits in individuals with chronic liver ailments (chronic hepatitis B, cirrhosis, HCC), scrutinizes microbiome alterations during the progression of these diseases, and assesses microbiome disparities among various Child-Pugh categories in cirrhosis sufferers.
A cohort of 60 CLD patients from the Third People's Hospital of Yunnan Province were recruited from February to August 2023, together with 37 healthy counterparts. Employing 16SrDNA high-throughput sequencing, we evaluated the diversity and composition of the gut microbiota.
Compared to healthy subjects, patients exhibited a reduced presence of and a corresponding decline in butyrate-producing genera. In contrast, an upsurge in was observed in the diseased cohorts, particularly an increase in that intensified with the disease's progression. At the genus level, the occurrence of , , , , and was higher, with numbers augmenting as the disease advanced. Furthermore, in cirrhosis patients, an increase in was noted as liver reserve diminished, alongside a decrease in and .
The reduced abundance of short-chain fatty acid-producing bacteria in the intestine, alongside the increased abundance of gram-negative bacteria such as and , may promote the progression of CLD.
在本研究中，我们致力于探索慢性肝病（CLD）三个不同阶段的肠道微生物群特征：慢性乙型肝炎、肝硬化和肝细胞癌（HCC）。
描绘慢性肝病（慢性乙型肝炎、肝硬化、HCC）患者的肠道微生物群特征，仔细研究这些疾病进展过程中的微生物群变化，并评估肝硬化患者不同Child-Pugh分级之间的微生物群差异。
2023年2月至8月，招募了云南省第三人民医院的60名CLD患者以及37名健康对照者。采用16SrDNA高通量测序技术，我们评估了肠道微生物群的多样性和组成。
与健康受试者相比，患者体内[具体菌属]的存在减少，产丁酸菌属相应减少。相比之下，在患病队列中观察到[另一具体菌属]增加，尤其是[某特定菌属]随着疾病进展而增加。在属水平上，[多个菌属名称]的发生率更高，随着疾病进展数量增加。此外，在肝硬化患者中，随着肝脏储备功能下降，[某菌属]增加，同时[另外两个菌属]减少。
肠道中产生短链脂肪酸的细菌丰度降低，以及诸如[某些革兰氏阴性菌属]等革兰氏阴性菌丰度增加，可能促进CLD的进展。
