Momin Md Sorique Aziz, Mohapatra Lishibanya
School of Physics and Astronomy, College of Science, Rochester Institute of Technology, Rochester, NY 14623, USA.
bioRxiv. 2025 May 28:2025.05.24.655867. doi: 10.1101/2025.05.24.655867.
How cytoskeletal structures control their assembly while sharing a common pool of their constituent parts is an open question in biology. Experiments indicate that mechanisms promoting the disassembly of these structures and replenishing the pool may play a vital role. Here, we compare the role of two modes of disassembly: monomer loss and loss of fragments (severing), in the assembly of bare filaments and bundles, modeled as a collection of linear filaments. Using analytical calculations and simulations, we show that severing can accelerate the assembly of these structures and ensure their precise size control in a shared pool. We also examine their length fluctuations and find that the decay in the autocorrelation function is faster with severing. Our study identifies parameters that influence assembly kinetics as well as the decay in autocorrelations of length fluctuations. These findings provide a framework for designing experiments that can differentiate between size control mechanisms in cytoskeletal structures.
细胞骨架结构如何在共享其组成部分的共同库的同时控制自身组装，这是生物学中的一个悬而未决的问题。实验表明，促进这些结构解体并补充该库的机制可能起着至关重要的作用。在这里，我们比较了两种解体模式的作用：单体损失和片段损失（切断），在裸丝和束的组装中，将其建模为线性丝的集合。通过分析计算和模拟，我们表明切断可以加速这些结构的组装，并确保它们在共享库中的精确尺寸控制。我们还研究了它们的长度波动，发现切断时自相关函数的衰减更快。我们的研究确定了影响组装动力学以及长度波动自相关衰减的参数。这些发现为设计能够区分细胞骨架结构中尺寸控制机制的实验提供了一个框架。
