Tian Ying, Xu Hangpeng, Yang Yanyan, Zhang Chuanxi, Chen Jianping, Li Junmin, Lu Jiabao
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
iScience. 2025 May 12;28(6):112640. doi: 10.1016/j.isci.2025.112640. eCollection 2025 Jun 20.
The secretions within the lateral oviduct lumen of the insect female reproductive tract (FRT) play crucial roles in reproduction, yet their protein composition and functional mechanisms remain poorly understood. In this study, we revealed that the lateral oviduct of the bean bug, , is composed of an outer muscle layer and an inner epithelium with a microvillar border. Proteomic analysis identified 159 FRT-highly expressed proteins, with functional characterization focusing on 10 putative secretory proteins, including 2 ovary-specific ( and ), 7 oviduct-specific ( and ), and 1 ovary-highly expressed () genes. Notably, RNAi-mediated knockdown of severely impaired choriogenesis, resulting in complete reproductive failure. These findings provide valuable insights into insect reproduction biology from the perspective of lateral oviduct-associated secretions and establish RpLoap2 as essential for successful reproduction in .
昆虫雌性生殖道（FRT）侧输卵管管腔内的分泌物在生殖过程中起着关键作用，但其蛋白质组成和功能机制仍知之甚少。在本研究中，我们发现豆蝽的侧输卵管由外层肌肉层和具有微绒毛边界的内层上皮组成。蛋白质组学分析鉴定出159种FRT高表达蛋白，功能表征聚焦于10种假定的分泌蛋白，包括2种卵巢特异性（和）、7种输卵管特异性（和）以及1种卵巢高表达（）基因。值得注意的是，RNA干扰介导的基因敲低严重损害了卵壳形成，导致完全生殖失败。这些发现从侧输卵管相关分泌物的角度为昆虫生殖生物学提供了有价值的见解，并确定RpLoap2对豆蝽成功繁殖至关重要。
