Jot Kiran, Kakkar Aanchal, Mishra Deepika, Roychoudhury Ajoy, Surya Varun, Sivakumar N, Nayyar Vivek
Department of Oral Pathology and Microbiology, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India.
Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
Indian J Otolaryngol Head Neck Surg. 2025 Jul;77(7):2562-2567. doi: 10.1007/s12070-025-05574-6. Epub 2025 May 22.
Oral squamous cell carcinoma (SCC) is the most common oral malignancy, representing up to 80-90% of all malignant neoplasms of the oral cavity. High-grade mucoepidermoid carcinoma (MEC) can share histological similarities with SCC, making their distinction challenging. SCC is characterized by frank anaplasia and evident keratinization, serving as distinguishing features from high-grade MEC. However, in incisional biopsies lacking these characteristics, differentiation becomes problematic, as immunohistochemical findings tend to be similar. Literature indicates that MUC5AC is present in MEC but notably absent in SCC. Thus, this study aims to evaluate MUC5AC expression in both OSCC and MEC and its role as a diagnostic immunomarker for these two entities. A comprehensive literature review revealed a scarcity of studies investigating MUC5AC expression in OSCC. Therefore, the current study seeks to investigate the immunoexpression of MUC5AC in OSCC and compare it with MEC. This study includes total of 40 cases, 20 cases of OSCC and 20 cases of MEC. Immunohistochemical analysis was performed to assess MUC5AC expression. The intensity and localization of immunoexpression were assessed in all cases. 95% MECs cases were immunopositive for MUC5AC. Moderate to strong cytoplasmic immunoreactivity for MUC5AC was noted, particularly in mucous cells present within the tissue section. In contrast, none of the 20 OSCC cases exhibited immunopositivity for MUC5AC. MUC5AC expression was predominantly confined to MEC, suggesting its potential utility as a biomarker for distinguishing between OSCC and MEC in differential diagnoses.
口腔鳞状细胞癌（SCC）是最常见的口腔恶性肿瘤，占口腔所有恶性肿瘤的80 - 90%。高级别黏液表皮样癌（MEC）在组织学上可能与SCC有相似之处，这使得二者的区分具有挑战性。SCC的特征是明显的间变和明显的角化，这是与高级别MEC的区别特征。然而，在缺乏这些特征的切开活检中，由于免疫组化结果往往相似，鉴别就成了问题。文献表明，MUC5AC存在于MEC中，但在SCC中明显缺失。因此，本研究旨在评估MUC5AC在口腔鳞状细胞癌（OSCC）和MEC中的表达及其作为这两种实体诊断免疫标志物的作用。全面的文献综述显示，研究OSCC中MUC5AC表达的研究很少。因此，本研究旨在调查MUC5AC在OSCC中的免疫表达，并将其与MEC进行比较。本研究共纳入40例病例，其中20例OSCC和20例MEC。进行免疫组化分析以评估MUC5AC的表达。评估所有病例免疫表达的强度和定位。95%的MEC病例MUC5AC免疫阳性。观察到MUC5AC有中度至强的细胞质免疫反应，特别是在组织切片中的黏液细胞中。相比之下，20例OSCC病例中无一例MUC5AC免疫阳性。MUC5AC表达主要局限于MEC，表明其在鉴别诊断中作为区分OSCC和MEC的生物标志物具有潜在用途。
