Han Jinhao, Ren Hongyu, Sun Mengjia, Li Mingxuan, Bo Cunyao, Fang Jiachuang, Wang Hongwei, Pang Yunlong, Wang Xiaoqian
National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, China.
Theor Appl Genet. 2025 Jun 12;138(7):148. doi: 10.1007/s00122-025-04933-9.
Wheat is one of the earliest domesticated crops and serves as a crucial staple food globally. However, farmland salinization severely restricts wheat production in many wheat-growing regions and poses a significant threat to global food security. In this study, Aegilops tauschii Y215, exhibiting superior salt tolerance, was selected from a panel of 91 Ae. tauschii lines. A set of wheat-Ae. tauschii introgression lines was developed through a cross between the wheat variety Jimai22 and Ae. tauschii accession Y215. The introgression lines were evaluated for salt tolerance at the seedling stage and genotyped using a 55 K SNP array. Phenotypic data, together with 2,727 high-quality polymorphic SNPs, were used to map quantitative trait loci (QTLs) associated with salt tolerance. A total of 14 QTLs were identified, including a major QTL, qSFWI6D, associated with shoot fresh weight. The favorable alleles at this locus were contributed by Ae. tauschii Y215. An introgression line, PT1047, exhibiting improved salt tolerance, was further utilized to construct a secondary segregation population for fine mapping of qSFWI6D. This effort narrowed the candidate region of qSFWI6D to a 205 Mb interval. These results demonstrate the potential of Ae. tauschii for improving wheat salt tolerance and provide valuable genetic resources for further breeding programs.
小麦是最早被驯化的作物之一，也是全球重要的主食。然而，农田盐碱化严重限制了许多小麦种植地区的小麦产量，对全球粮食安全构成重大威胁。在本研究中，从91份节节麦材料中筛选出耐盐性优异的节节麦Y215。通过小麦品种济麦22与节节麦Y215杂交，构建了一套小麦-节节麦渐渗系。对渐渗系进行苗期耐盐性评价，并利用55K SNP芯片进行基因分型。结合表型数据和2727个高质量多态性SNP，定位与耐盐性相关的数量性状位点（QTL）。共鉴定出14个QTL，其中包括一个与地上部鲜重相关的主效QTL qSFWI6D。该位点的有利等位基因来自节节麦Y215。进一步利用耐盐性提高的渐渗系PT1047构建次级分离群体，对qSFWI6D进行精细定位。将qSFWI候选区域缩小到205 Mb区间。这些结果证明了节节麦在提高小麦耐盐性方面的潜力，并为进一步的育种计划提供了宝贵的遗传资源。
