Hou Zishuo, Zhang Wuyang, Li Gangfeng, Cui Hannan, Chen Hongli, Wang Tengjiao, Feng Yafei, Li Peng
State Key Laboratory of Flexible Electronics (LOFE) & Institute of Flexible Electronics (IFE), Shaanxi Key Laboratory of Flexible Electronics & MIIT Key Laboratory of Flexible Electronics (KLoFE), Xi'an Institute of Biomedical Materials and Engineering (IBME), Northwestern Polytechnical University (NPU), Xi'an, Shaanxi 710072, P. R. China.
Department of Orthopedics, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P. R. China.
ACS Appl Mater Interfaces. 2025 Jun 25;17(25):36497-36510. doi: 10.1021/acsami.5c07099. Epub 2025 Jun 12.
Infected diabetic wounds have become a major global health threat due to their complex conditions, making them difficult to heal. Herein, a coaxial fibrous dressing capable of sequential release of nitric oxide (NO) and molecular hydrogen (H) was designed for the treatment of infected diabetic wounds. The outer layer of the coaxial fibers was physically loaded with the NO donor, enabling the first stage of rapid and high-level NO release (over 170 μM). The released NO contributed to the antibacterial function of the dressing ( antibacterial assays demonstrated eradication rates of 99.99% and 99.04% against and , respectively), thus facilitating accelerated transition of the wound through the inflammatory phase. The inner layer of coaxial fibers becomes progressively exposed following structural disintegration, enabling the hydrolysis of encapsulated H donor to achieve on-demand release of H under near-infrared (NIR) irradiation. The released H could promote wound healing through reactive oxygen species (ROS) scavenging and macrophage M2 polarization, as validated by cell assessments. infected full-thickness skin defect model in diabetic mice demonstrated that the NO/H sequential release coaxial fibrous dressing significantly accelerated healing of infected wounds through rapid bactericidal action via substantial NO release, followed by further sequential H release promoting repairing and regeneration. This temporally sequential delivery of antibacterial and healing-promoting functions significantly accelerated the healing of infected diabetic wounds.
由于感染性糖尿病伤口状况复杂、难以愈合，已成为全球主要的健康威胁。在此，设计了一种能够依次释放一氧化氮（NO）和分子氢（H₂）的同轴纤维敷料，用于治疗感染性糖尿病伤口。同轴纤维的外层物理负载了NO供体，实现了第一阶段快速、高水平的NO释放（超过170μM）。释放的NO有助于敷料发挥抗菌功能（抗菌试验表明，对金黄色葡萄球菌和大肠杆菌的根除率分别为99.99%和99.04%），从而促进伤口加速度过炎症期。随着结构解体，同轴纤维的内层逐渐暴露，使得封装的H₂供体水解，在近红外（NIR）照射下实现H₂的按需释放。如细胞评估所证实，释放的H₂可通过清除活性氧（ROS）和促进巨噬细胞M2极化来促进伤口愈合。糖尿病小鼠的感染性全层皮肤缺损模型表明，NO/H₂依次释放的同轴纤维敷料通过大量释放NO实现快速杀菌作用，随后进一步依次释放H₂促进修复和再生，显著加速了感染伤口的愈合。这种抗菌和促进愈合功能的时间顺序递送显著加速了感染性糖尿病伤口的愈合。
