Arioni Sol, Huk Marlene, Batista Pablo R, Arias Daniel O, Gobello Cristina, Blanco Paula G
Center of Reproductive Physiology and Diagnostic Imaging, Faculty of Veterinary Sciences of the National University of La Plata, Argentina; CONICET, Argentina.
Center of Reproductive Physiology and Diagnostic Imaging, Faculty of Veterinary Sciences of the National University of La Plata, Argentina; CONICET, Argentina.
Theriogenology. 2025 Oct 1;245:117526. doi: 10.1016/j.theriogenology.2025.117526. Epub 2025 Jun 5.
This study aimed to describe and compare uterine artery velocimetry in bitches suffering from endometritis, cystic endometrial hyperplasia (CEH) and healthy animals, and to evaluate the effect of clinical, ultrasonographic, bacteriological and histopathological parameters on the uterine artery resistance index in these bitches. According to clinical, two-dimensional ultrasonographic, gross and histopathological uterine evaluation, thirty-three bitches were classified as: Endometritis (END; n = 13), CEH (n = 7) and healthy bitches (NG; n = 13). Uterine ultrasonographic examinations were performed on each patient using B mode and pulsed-wave Doppler. Peak systolic velocity (PSV), end diastolic velocity (EDV) and resistance index (RI) were obtained. A generalized linear model was carried out to analyze the effect of group, clinical, ultrasonographic, bacteriological and histopathological variables on PSV, EDV and RI. EDV was higher in CEH than END (P < 0.05) and NG (P < 0.01). No difference was observed between END and NG (P > 0.1). END presented a lower RI than NG and higher RI than CEH (P < 0.01). Additionally, CEH showed lower RI than NG (P < 0.01). This study showed an effect of higher values of endometrium thickness, PMNN/HPF and vascular congestion on RI (P < 0.01). It was concluded that, in these bitches, uterine artery RI was a valuable parameter to distinguish endometritis from normal uterus and CEH. An increase in neutrophils and vascular congestion influenced RI, remarking the importance of angiogenesis and the inflammatory reaction on uterine perfusion increase. This study is the first to identify an ultrasound parameter that differentiates endometritis from normal uterus in dogs.
本研究旨在描述和比较患有子宫内膜炎、囊性子宫内膜增生（CEH）的母犬以及健康动物的子宫动脉血流速度测定情况，并评估临床、超声、细菌学和组织病理学参数对这些母犬子宫动脉阻力指数的影响。根据临床、二维超声、大体和组织病理学对子宫的评估，33只母犬被分为：子宫内膜炎组（END；n = 13）、CEH组（n = 7）和健康母犬组（NG；n = 13）。对每只母犬使用B模式和脉冲波多普勒进行子宫超声检查。获得收缩期峰值流速（PSV）、舒张末期流速（EDV）和阻力指数（RI）。采用广义线性模型分析组、临床、超声、细菌学和组织病理学变量对PSV、EDV和RI的影响。CEH组的EDV高于END组（P < 0.05）和NG组（P < 0.01）。END组和NG组之间未观察到差异（P > 0.1）。END组的RI低于NG组且高于CEH组（P < 0.01）。此外，CEH组的RI低于NG组（P < 0.01）。本研究表明，子宫内膜厚度、每高倍视野多形核中性粒细胞（PMNN/HPF）和血管充血值较高对RI有影响（P < 0.01）。得出的结论是，在这些母犬中，子宫动脉RI是区分子宫内膜炎与正常子宫和CEH的一个有价值的参数。中性粒细胞增加和血管充血影响RI，表明血管生成和炎症反应对子宫灌注增加的重要性。本研究首次确定了一种能区分犬子宫内膜炎与正常子宫的超声参数。
