Fotouh Ahmed, Elbarbary Nady Khairy, Momenah Maha Abdullah, Khormi Mohsen A, Mohamed Wafaa H, Sherkawy Hoda S, Ahmed Ahmed Ezzat, Diab Mohamed, Elshafae Said
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, New Valley University, El Kharga, Egypt.
MBA Program, Department of Business Administration, Marywood University, Scranton, PA.
Am J Vet Res. 2025 Jun 9;86(8). doi: 10.2460/ajvr.25.03.0074. Print 2025 Aug 1.
To evaluate the therapeutic potential of mesenchymal stem cells (MSCs) in alleviating carbon tetrachloride (CCl4)-induced liver damage in rats.
The study was conducted from October 2024 through February 2025. A total of 80 rats (20 rats for MSC isolation and 60 additional rats) were divided into groups. The negative control groups received olive oil twice weekly for 9 weeks, a positive control group received CCl4 in olive oil twice weekly for 9 weeks, and an MSC test group received the same treatment as the positive control group, followed by treatment with MSCs once a week for 4 weeks. Liver enzyme levels (ALT, ASP, and ALP) and the oxidant-antioxidant parameters (glutathione peroxidase, superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase activity, and total antioxidant capacity) were assessed. Histopathological analysis of liver tissues was performed to examine the extent of liver damage.
The results showed increases in liver enzymes and oxidative stress markers in the positive control group compared to the negative control group. The MSC test group exhibited reduced liver enzyme levels, enhanced antioxidant activity, and improved histological findings, with reduced inflammation and fibrosis.
MSCs possess a potent hepatoprotective effect through the restoration of antioxidant defenses, reduction of oxidative stress, and promotion of tissue regeneration.
This study supports the potential of MSC-based therapy as a promising approach for the treatment of liver damage caused by toxic insults, such as CCl4.
评估间充质干细胞（MSCs）在减轻四氯化碳（CCl4）诱导的大鼠肝损伤方面的治疗潜力。
该研究于2024年10月至2025年2月进行。总共80只大鼠（20只用于分离MSCs，另外60只）被分组。阴性对照组每周两次接受橄榄油，共9周；阳性对照组每周两次接受溶于橄榄油的CCl4，共9周；MSCs试验组接受与阳性对照组相同的处理，随后每周一次接受MSCs治疗，共4周。评估肝酶水平（谷丙转氨酶、谷草转氨酶和碱性磷酸酶）以及氧化-抗氧化参数（谷胱甘肽过氧化物酶、超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶、过氧化氢酶活性和总抗氧化能力）。对肝组织进行组织病理学分析以检查肝损伤程度。
结果显示，与阴性对照组相比，阳性对照组的肝酶和氧化应激标志物增加。MSCs试验组肝酶水平降低，抗氧化活性增强，组织学结果改善，炎症和纤维化减轻。
MSCs通过恢复抗氧化防御、降低氧化应激和促进组织再生具有强大的肝保护作用。
本研究支持基于MSCs的治疗作为治疗由毒性损伤如CCl4引起的肝损伤的一种有前景方法的潜力。
