Kılıçkaya Selvi Emine
Department of Chemistry and Chemical Processing Technologies, Mustafa Çıkrıkcıoglu Vocational School, Kayseri University, Kayseri, 38280, Türkiye, Turkey.
BMC Chem. 2025 Jun 12;19(1):165. doi: 10.1186/s13065-025-01539-6.
Diabetes mellitus is a global health problem leading to various complications associated with high blood sugar levels. One of the alternative ways to manage the disease in traditional medicine is herbal therapy. Prunus divaricata L has been used in this regard in traditional medicine. The aim of the study was to investigate the antidiabetic, cytotoxic, antioxidant activity and phenolic profile of its fruit extract by spectroscopic and chromatographic methods. The methanol extract exhibited strong activity on DPPH radical scavenging, FRAP and CUPRAC assays, (0.023 mg/mL, 1.02 µmol trolox/mg dry ext, and 5.53 µmol trolox/mg dry ext, respectively). The antidiabetic activity was evaluated on α-glucosidase and α-amylase. The extract showed considerable activity on α-glucosidase (98.64% inhibition at 100 µg/mL). The anticancer potential of extract was evaluated using MCF-7 (ER + breast) cell line. The extract significantly decreased cell viability in MCF-7 cell line at a concentration of 3.2 µg/mL. In parallel to the biological activity tests, phytochemical composition of the extract was also studied. In addition to the qualitative chromatographic analysis, quantification of eight individual compounds was also determined in the extract. Results indicate that methanol extract contained considerable amounts of p-coumaric acid and ferulic acid along with chlorogenic acid, caffeic acid, rutin, quercetin, apigenin and kaempferol. Prunus divaricata L. fruit extract may protect against damage caused by oxidative stress by increasing antioxidant capacity in diabetes.
糖尿病是一个全球性的健康问题，会导致各种与高血糖水平相关的并发症。传统医学中治疗该疾病的一种替代方法是草药疗法。欧李在传统医学中已被用于此方面。本研究的目的是通过光谱和色谱方法研究其果实提取物的抗糖尿病、细胞毒性、抗氧化活性和酚类成分。甲醇提取物在DPPH自由基清除、FRAP和CUPRAC测定中表现出较强的活性（分别为0.023 mg/mL、1.02 μmol Trolox/mg干提取物和5.53 μmol Trolox/mg干提取物）。对α-葡萄糖苷酶和α-淀粉酶进行了抗糖尿病活性评估。提取物对α-葡萄糖苷酶表现出相当强的活性（在100 μg/mL时抑制率为98.64%）。使用MCF-7（雌激素受体阳性乳腺癌）细胞系评估了提取物的抗癌潜力。提取物在3.2 μg/mL的浓度下显著降低了MCF-7细胞系的细胞活力。在进行生物活性测试的同时，还研究了提取物的植物化学成分。除了定性色谱分析外，还对提取物中的八种单一化合物进行了定量测定。结果表明，甲醇提取物含有大量的对香豆酸和阿魏酸，以及绿原酸、咖啡酸、芦丁、槲皮素、芹菜素和山奈酚。欧李果实提取物可能通过提高糖尿病患者的抗氧化能力来预防氧化应激造成的损害。
