Drosdzol-Cop Agnieszka, Skrzypulec-Plinta Violetta
Woman's Health Institute and Medical University of Silesia, Katowice, Poland.
J Obstet Gynaecol Res. 2012 Oct;38(10):1245-53. doi: 10.1111/j.1447-0756.2012.01860.x. Epub 2012 May 8.
The aim of this study was to determine the role of serum and peritoneal interleukin (IL)-6, tumor necrosis factor (TNF)-α and glycodelin A levels as diagnostic markers of endometriosis in adolescent girls.
The study encompassed 50 adolescent girls, aged 13-19 years, after menarche and with chronic pelvic pain who qualified for diagnostic laparoscopy. The patients were allocated into two groups: group I (endometriosis group) consisted of subjects with diagnosed endometriosis (n = 33, 66%) and group II (control group) included those whose laparoscopic examinations revealed no evidence of endometriosis (n = 17, 34%). IL-6, TNF-α and glycodelin A concentrations in serum and peritoneal samples were assessed using commercially available human enzyme-linked immunosorbent assay kits. The value of P < 0.05 was adopted as the level of statistical significance.
Compared with the control group, adolescent girls with endometriosis had significantly higher peritoneal fluid levels of: IL-6 (525.10 ± 1168.53 pg/mL vs 62.96 ± 82.35 pg/mL), TNF-α (5.79 ± 5.60 pg/mL vs 1.68 ± 1.24 pg/mL) and glycodelin A (94.24 ± 60.97 ng/mL vs 53.52 ± 41.43 ng/mL). Peritoneal IL-6, TNF-α and glycodelin A provided a good method of discrimination between subjects with endometriosis and controls. Using cut-off points for peritoneal fluid IL-6 (90.00 pg/mL), TNF-α (3.00 pg/mL) and glycodelin A (60.0 ng/mL), exceptionally high odds ratios (10.2; 14.6; 2.2) were obtained in the prediction of endometriosis in adolescents.
At the cut-off value of 3.00 pg/mL, peritoneal TNF-α can be a reliable screening marker for the prediction of endometriosis in adolescents, giving a 14.6-fold higher probability of endometriosis detection in girls with chronic pelvic pain.
本研究旨在确定血清和腹腔白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α及糖蛋白A水平作为青春期女孩子宫内膜异位症诊断标志物的作用。
本研究纳入了50名初潮后年龄在13 - 19岁、患有慢性盆腔疼痛且符合诊断性腹腔镜检查条件的青春期女孩。患者被分为两组：第一组（子宫内膜异位症组）由诊断为子宫内膜异位症的受试者组成（n = 33，66%），第二组（对照组）包括腹腔镜检查未发现子宫内膜异位症证据的患者（n = 17，34%）。使用市售的人酶联免疫吸附测定试剂盒评估血清和腹腔样本中IL-6、TNF-α及糖蛋白A的浓度。采用P < 0.05作为统计学显著性水平。
与对照组相比，患有子宫内膜异位症的青春期女孩腹腔液中IL-6（525.10 ± 1168.53 pg/mL对62.96 ± 82.35 pg/mL）、TNF-α（5.79 ± 5.60 pg/mL对1.68 ± 1.24 pg/mL）和糖蛋白A（94.24 ± 60.97 ng/mL对53.52 ± 41.43 ng/mL）水平显著更高。腹腔IL-6、TNF-α和糖蛋白A为区分子宫内膜异位症患者和对照组提供了一种良好的方法。使用腹腔液IL-6（90.00 pg/mL）、TNF-α（3.00 pg/mL）和糖蛋白A（60.ng/mL）的截断点，在预测青春期子宫内膜异位症时获得了极高的优势比（10.2；14.6；2.2）。
在截断值为3.00 pg/mL时，腹腔TNF-α可作为预测青春期子宫内膜异位症的可靠筛查标志物，使慢性盆腔疼痛女孩中子宫内膜异位症的检出概率提高14.6倍。
