Murakami Ryuta, Chiba Yoichi, Takebayashi Genta, Wakamatsu Keiji, Miyai Yumi, Matsumoto Koichi, Uemura Naoya, Yanase Ken, Ogino Yuichi, Ueno Masaki
Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Takamatsu 761-0793, Kagawa, Japan.
Department of Anesthesiology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Takamatsu 761-0793, Kagawa, Japan.
Int J Mol Sci. 2025 May 27;26(11):5130. doi: 10.3390/ijms26115130.
The functional significance of the choroid plexus (CP), such as in the control of circadian rhythm as well as production of cerebrospinal fluid, is attracting attention. Transepithelial and junctional transport between epithelial cells of CP plays an important role in its function. Recently, an epithelial cadherin, E-cadherin, as well as non-epithelial cadherins were confirmed to be expressed in CP epithelial cells. The serine protease inhibitor Kunitz type 1 (SPINT1) is expressed in many kinds of epithelial cells and affects epithelial developmental function by controlling E-cadherin expression. However, it has not been confirmed whether SPINT1 is expressed in epithelial cells of CP. Thus, in this study, we examined whether SPINT1 is expressed in CP epithelial cells by immunohistochemistry and RT-PCR. Immunohistochemical expression of SPINT1 was noted in the cytoplasm of epithelial cells of humans and mice, and mRNA of SPINT1 was expressed in samples derived from the CP of mice. SPINT1 was typically expressed in CP epithelial cells with E-cadherin and Smad-interacting protein (SIP1), an E-cadherin transcriptional repressor. Some enlarged epithelial cells showed strong SPINT1 signals. These findings indicate that SPINT1 is expressed in epithelial cells of CP in relation to E-cadherin expression.
脉络丛（CP）的功能意义，如在昼夜节律控制以及脑脊液产生方面，正受到关注。CP上皮细胞之间的跨上皮和连接运输在其功能中起着重要作用。最近，一种上皮钙黏蛋白E-钙黏蛋白以及非上皮钙黏蛋白被证实可在CP上皮细胞中表达。丝氨酸蛋白酶抑制剂库尼茨1型（SPINT1）在多种上皮细胞中表达，并通过控制E-钙黏蛋白的表达影响上皮发育功能。然而，尚未证实SPINT1是否在CP上皮细胞中表达。因此，在本研究中，我们通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测SPINT1是否在CP上皮细胞中表达。在人和小鼠上皮细胞的细胞质中观察到SPINT1的免疫组织化学表达，并且在源自小鼠CP的样本中表达了SPINT1的信使核糖核酸（mRNA）。SPINT1通常在CP上皮细胞中与E-钙黏蛋白和E-钙黏蛋白转录抑制因子Smad相互作用蛋白（SIP1）共同表达。一些增大的上皮细胞显示出强烈的SPINT1信号。这些发现表明SPINT1在CP上皮细胞中的表达与E-钙黏蛋白的表达相关。
