Hu Jun, Hu Jinxue, Duan Shaoguang, Xiang Congchao, Duan Yanfeng, Zhang Shuqing, Li Guangcun
State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang 050041, China.
Int J Mol Sci. 2025 May 30;26(11):5278. doi: 10.3390/ijms26115278.
The network regulatory mechanism governing the dynamics of plant maturity and tuber development in potatoes ( L.) has remained a major focus in potato molecular biology research. In this study, three potato cultivars with different maturity periods ('Shishu 2', 'Zhongshu 3', and 'Zhongshu 49') were examined. RNA sequencing was performed on samples from five tissues, including the leaves, stems, stolon (T0), sub-apical swellings (T1), and initiation stage (T2), to reveal the co-expression regulatory network involved in leaf, stem, and tuber development. and were significantly upregulated in the early-maturing cultivar 'Shishu 2'. Differentially expressed genes were classified into 18 modules (ME) using weighted gene co-expression network analysis (WGCNA). Among these, ME1, ME3, and ME13 showed significant positive correlations with leaf tissue, ME2, ME4, and ME15 with stem tissue, and ME7, ME8, and ME14 with T1 and T2 tissues. was identified as the core hub gene of ME4. Genes such as , , and exhibited significant co-expression in leaf-related modules. and played important regulatory roles in linking the expression networks of stems and tubers. Metabolism-related genes, including / and , were also found to be crucial in mediating interactions between leaf and tuber tissues. Therefore, this study provides new insights into the regulatory network governing tuberous signal transmission from leaves and stems to tubers.
调控马铃薯（Solanum tuberosum L.）植株成熟和块茎发育动态的网络调控机制一直是马铃薯分子生物学研究的主要焦点。在本研究中，对三个不同成熟期的马铃薯品种（‘实薯2号’、‘中薯3号’和‘中薯49号’）进行了研究。对来自叶片、茎、匍匐茎（T0）、亚顶端膨大部位（T1）和起始阶段（T2）这五个组织的样本进行了RNA测序，以揭示参与叶片、茎和块茎发育的共表达调控网络。[具体基因1]和[具体基因2]在早熟品种‘实薯2号’中显著上调。使用加权基因共表达网络分析（WGCNA）将差异表达基因分为18个模块（ME）。其中，ME1、ME3和ME13与叶片组织呈显著正相关，ME2、ME4和ME15与茎组织呈显著正相关，ME7、ME8和ME14与T1和T2组织呈显著正相关。[具体基因3]被鉴定为ME4的核心枢纽基因。[具体基因4]、[具体基因5]和[具体基因6]等基因在叶片相关模块中表现出显著的共表达。[具体基因7]和[具体基因8]在连接茎和块茎的表达网络中发挥重要调控作用。还发现包括[具体基因9]/[具体基因10]和[具体基因11]在内的代谢相关基因在介导叶片和块茎组织之间的相互作用中至关重要。因此，本研究为调控从叶片和茎到块茎的块茎信号传递的调控网络提供了新的见解。
