Zhao Jinfa, Chen Yuan, Wang Jiajun, Wang Chunqing, Zhou Yan
Integrative Science Center of Germplasm Creation in Western China (Chongqing), Science City/Citrus Research Institute, Southwest University, Chongqing 400712, China.
Institute of Plant Protection, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
Plants (Basel). 2025 May 27;14(11):1629. doi: 10.3390/plants14111629.
-mediated transformation systems using epicotyl explants have been widely used for genetic transformations of citrus. However, their application in lemons is severely constrained by browning of epicotyl tissues, which leads to an extremely low efficiency of transformation. In this study, we developed an optimized -mediated transformation system using whole cotyledonary node explants of 'Eureka' lemon (), which significantly reduced tissue browning and enhanced transformation efficiency up to 14.48%. In addition, preparation of the whole cotyledonary node was simple and rapid, which reduced time and labor. This system facilitated efficient generation of transgenic lemon plantlets and provided a novel explant source for citrus transformation.
利用上胚轴外植体的介导转化系统已被广泛用于柑橘的遗传转化。然而，它们在柠檬中的应用受到上胚轴组织褐变的严重限制，这导致转化效率极低。在本研究中，我们开发了一种优化的介导转化系统，使用“尤力克”柠檬（）的完整子叶节外植体，该系统显著减少了组织褐变，并将转化效率提高到了14.48%。此外，完整子叶节的制备简单快速，减少了时间和劳动力。该系统促进了转基因柠檬植株的高效产生，并为柑橘转化提供了一种新的外植体来源。
