Bao Jiying, Gou Huimin, Yang Shangwen, Liang Guoping, Mao Juan
The College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel). 2025 May 30;14(11):1672. doi: 10.3390/plants14111672.
The CorA/MRS2-type transporters represent a crucial family of magnesium ion transporters widely distributed in plants. Through comprehensive screening and alignment using the Phytozome database, we identified seven magnesium-related Confirm the deletion of the "Chinese Province" column in the address. genes in apple ( to ), which were distributed across seven distinct chromosomes. Phylogenetic analysis classified these genes into five distinct clades. Tissue-specific expression profiles revealed the differential expression patterns of members in different tissues such as the apple roots, stems, leaves, seedlings, seeds, flowers, and fruits. Among them, the expression level of was the highest in fruits, while that of was the lowest in seeds. Analysis of -regulatory elements in promoter regions identified numerous light-responsive elements, MYB binding sites, and hormone-responsive elements, suggesting their transcriptional regulation may be influenced by related metabolic pathways or signaling molecules. qRT-PCR results showed that the relative expression levels of all genes were significantly upregulated compared with CK under M3 treatment, while there were no significant differences in other treatments. Among them, the upregulation of was the most significant, increasing by 142% compared with CK. Notably, all genes were significantly upregulated under 4 mmol·L MgSO treatment. Subcellular localization experiments conducted in tobacco leaves confirmed the membrane and cytoplasmic distribution of these transporters, consistent with bioinformatic predictions. These genes may become candidate genes for subsequent functional studies. This work will provide a basis for future research on the response mechanism and function of the MRS2 gene family in response to magnesium stress.
CorA/MRS2型转运蛋白是广泛分布于植物中的一类关键的镁离子转运蛋白家族。通过使用植物基因组数据库进行全面筛选和比对，我们在苹果中鉴定出7个与镁相关的基因（至），它们分布在7条不同的染色体上。系统发育分析将这些基因分为5个不同的进化枝。组织特异性表达谱揭示了这些成员在苹果根、茎、叶、幼苗、种子、花和果实等不同组织中的差异表达模式。其中，在果实中的表达水平最高，而在种子中的表达水平最低。对启动子区域的调控元件分析鉴定出许多光响应元件、MYB结合位点和激素响应元件，表明它们的转录调控可能受相关代谢途径或信号分子的影响。qRT-PCR结果显示，与对照相比，在M3处理下所有基因的相对表达水平均显著上调，而在其他处理中无显著差异。其中，的上调最为显著，与对照相比增加了142%。值得注意的是，在4 mmol·L MgSO处理下所有基因均显著上调。在烟草叶片中进行的亚细胞定位实验证实了这些转运蛋白的膜和细胞质分布，与生物信息学预测一致。这些基因可能成为后续功能研究的候选基因。这项工作将为未来研究MRS2基因家族响应镁胁迫的机制和功能提供依据。
