Casella Claudio, Cornelli Umberto, Ballaz Santiago, Zanoni Giuseppe, Ramos-Guerrero Luis
Department of Chemistry, University of Pavia, 27100 Pavia, Italy.
School of Medicine, Loyola University, Chicago 60660, IL, USA.
Polymers (Basel). 2025 May 23;17(11):1445. doi: 10.3390/polym17111445.
Chitosan is recognized by its capacity to bind lipids based on the viscosity and degree of deacetylation. We analyzed the in vitro binding of chitosan (PCC) with extra virgin olive oil and sunflower oil at temperatures and pH levels that approximate gastric-like conditions. In the tube test, 4 mg of PCC and 0.3 g of either EVO or of SO oils were mixed by stirring in test tubes with 4 mL of water acidified with HCl to a pH of 3. The PCC binding capability was determined by measuring the differences between the suspension without PCC and the suspension with PCC added. A scanning electron microscope (SEM) was utilized to further observe the uniformity and morphology of the emulsified PCC/oil hydrogels. In the test tube, PCC was shown to have a 1:11 () binding capacity for EVO and 1:15 () for SO. The SEM-based examination demonstrated a smooth surface with fine porosity of the microstructure of either PCC/oil hydrogel, proving successful emulsification. Under conditions similar to those in the stomach after a meal, including acidity, mixed composition, and temperature, PCC efficiently binds and emulsifies EVO and SO.
壳聚糖因其基于粘度和脱乙酰度结合脂质的能力而被认可。我们分析了壳聚糖（PCC）在接近胃内环境的温度和pH值条件下与特级初榨橄榄油和葵花籽油的体外结合情况。在试管试验中，将4毫克PCC与0.3克特级初榨橄榄油或葵花籽油在装有4毫升用盐酸酸化至pH值为3的水的试管中搅拌混合。通过测量未添加PCC的悬浮液与添加PCC后的悬浮液之间的差异来确定PCC的结合能力。利用扫描电子显微镜（SEM）进一步观察乳化的PCC/油水凝胶的均匀性和形态。在试管中，PCC对特级初榨橄榄油的结合能力为1:11（），对葵花籽油为1:15（）。基于SEM的检查表明，两种PCC/油水凝胶的微观结构表面光滑且孔隙细小，证明乳化成功。在类似于饭后胃内的条件下，包括酸度、混合成分和温度，PCC能有效地结合并乳化特级初榨橄榄油和葵花籽油。
