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苏里南和印度尼西亚蛇类中不同物种的分子检测

Molecular Detection of Different Species of in Snakes from Surinam and Indonesia.

作者信息

Polláková Magdaléna, Sučik Monika, Petrilla Vladimír

机构信息

Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.

Zoological Department, Zoological Garden Košice, Široká 31, Kavečany, 040 06 Košice, Slovakia.

出版信息

Animals (Basel). 2025 May 26;15(11):1556. doi: 10.3390/ani15111556.

Abstract

In recent decades, the keeping of exotic animals has gained popularity among enthusiasts worldwide. However, alongside the development of exotic animal husbandry, issues related to health status and adequate veterinary care are coming to the forefront. The introduction of new snakes into a collection and shared enclosures should always be preceded by an assessment of their parasitic status. In our study, we present an overview of the screening for the presence of spp. in individuals captured in regions of Indonesia and Suriname, intended for further trade. Out of 40 tested fecal samples, the presence of cryptosporidial oocysts was confirmed in 6 samples. Detection was performed by molecular methods, namely Nested PCR targeting the gene region (60 kDa glycoprotein). By sequencing, we confirmed the presence of in ( = 1) and ( = 1), in spp. ( = 2), and in spp. ( = 2), which is the very first time that this species has been detected in snakes in captivity. Although the presence of species, typical for snakes, was not detected, the identified species may pose a health risk to humans, especially workers who come into direct contact with animals.

摘要

近几十年来,饲养外来动物在全球爱好者中越来越流行。然而,随着外来动物养殖业的发展,与健康状况和充分兽医护理相关的问题日益凸显。在将新蛇引入收藏和共享围栏之前,应始终先评估它们的寄生虫状况。在我们的研究中,我们概述了对印度尼西亚和苏里南地区捕获的、拟用于进一步贸易的个体进行 spp. 检测的情况。在 40 份检测的粪便样本中,有 6 份样本确认存在隐孢子虫卵囊。检测采用分子方法,即针对 基因区域(60 kDa 糖蛋白)的巢式 PCR。通过测序,我们确认在 ( = 1)和 ( = 1)中存在 ,在 spp. ( = 2)中存在 ,以及在 spp. ( = 2)中存在 ,这是该物种首次在圈养蛇类中被检测到。尽管未检测到蛇类中典型的 物种,但已鉴定出的物种可能对人类,尤其是与动物直接接触的工作人员构成健康风险。

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Molecular identification of Cryptosporidium spp. in pet snakes in Beijing, China.中国北京宠物蛇种隐孢子虫的分子鉴定。
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