Lahteenmaki P, Marrs R P, Schechter J, Zacharias S, Kletzky O A
Am J Obstet Gynecol. 1985 Oct 15;153(4):349-57. doi: 10.1016/0002-9378(85)90070-5.
Bromocriptine in concentrations up to 5 X 10(-4) mol/L was studied for any deleterious effects upon normal rat pituitary cells, as well as on the rat GH3 cell line. Normal rat pituitary glands were obtained by decapitation from 50-day-old female Wistar rats and dispersed with 0.25% trypsin. The cells (10(5) per plate) were then incubated in 60 by 15 mm plates (Falcon) that contained 3 ml of Dulbecco's modified Eagle's medium with 10% fetal calf serum. GH3 cells were plated in a similar fashion. Bromocriptine was added in concentrations of 5 X 10(-4) to 5 X 10(-9) mol/L, and aliquots of medium were obtained at 6, 24, and 48 hours for the determination of growth hormone and prolactin. Cell counts were performed at 24 and 48 hours. A significant reduction in concentrations of growth hormone and prolactin was observed with concentrations of bromocriptine of 5 X 10(-5) and 5 X 10(-4) mol/L at 24 and 48 hours (p less than 001). Although no significant changes in cell counts were observed in the normal rat pituitary cells, the GH3 cells showed complete disruption at 48 hours only in the plates that contained the highest concentrations of bromocriptine. Electron microscopy of normal rat cells and GH3 demonstrated selective cytotoxic effects only on the GH3 cells. In conclusion, bromocriptine has been demonstrated to have a direct effect on hormone release and on the morphologic characteristics of tumor cells but not normal pituitary cells.
研究了浓度高达5×10⁻⁴mol/L的溴隐亭对正常大鼠垂体细胞以及大鼠GH3细胞系的任何有害影响。通过断头法从50日龄雌性Wistar大鼠获取正常大鼠垂体，并用0.25%胰蛋白酶进行分散。然后将细胞（每板10⁵个）接种于60×15mm的培养板（Falcon）中，培养板中含有3ml添加10%胎牛血清的杜尔贝科改良伊格尔培养基。GH3细胞以类似方式接种。加入浓度为5×10⁻⁴至5×10⁻⁹mol/L的溴隐亭，并在6小时、24小时和48小时获取培养基 aliquots 用于测定生长激素和催乳素。在24小时和48小时进行细胞计数。在24小时和48小时时，观察到溴隐亭浓度为5×10⁻⁵和5×10⁻⁴mol/L时，生长激素和催乳素浓度显著降低（p＜0.001）。虽然正常大鼠垂体细胞的细胞计数未观察到显著变化，但GH3细胞仅在含有最高浓度溴隐亭的培养板中在48小时时出现完全破坏。对正常大鼠细胞和GH3细胞的电子显微镜检查表明，仅对GH3细胞有选择性细胞毒性作用。总之，已证明溴隐亭对激素释放以及肿瘤细胞的形态特征有直接影响，但对正常垂体细胞无影响。
