Liu Ce, He Wanchong
College of Science, Yanbian University, Yanji 133002, China.
Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252000, China.
Molecules. 2025 May 29;30(11):2375. doi: 10.3390/molecules30112375.
Aptamers are synthetic nucleic acids or peptides that exhibit high specificity and affinity for target molecules such as small molecules, proteins, or cells. Due to their ability to bind precisely to these targets, aptamers have found widespread use in bioanalytical and diagnostic applications. Rolling circle amplification (RCA) is an amplification technique that utilizes DNA or RNA templates, where circular primers are extended by polymerases to generate multiple repeated sequences, enabling highly sensitive detection of target molecules. The integration of aptamers with RCA offers significant advantages, enhancing both the specificity and sensitivity of detection while ensuring a fast and straightforward process. This synergy has already been widely applied across various fields, including fluorescence, microfluidics, visualization, and electrochemical technologies. Examples include molecular probe development, rapid detection of disease biomarkers, and environmental monitoring. Looking ahead, the aptamer-RCA platform holds great promise for advancing early disease diagnosis, precision medicine, and the development of nanosensors, driving innovation and new applications in these fields.
适体是对小分子、蛋白质或细胞等靶分子具有高特异性和亲和力的合成核酸或肽。由于适体能够精确结合这些靶标，因此在生物分析和诊断应用中得到了广泛应用。滚环扩增（RCA）是一种利用DNA或RNA模板的扩增技术，其中环形引物由聚合酶延伸以产生多个重复序列，从而实现对靶分子的高灵敏度检测。适体与RCA的结合具有显著优势，在确保检测过程快速直接的同时，提高了检测的特异性和灵敏度。这种协同作用已经在包括荧光、微流体、可视化和电化学技术在内的各个领域得到广泛应用。例子包括分子探针开发、疾病生物标志物的快速检测和环境监测。展望未来，适体-RCA平台在推进早期疾病诊断、精准医学和纳米传感器开发方面具有巨大潜力，将推动这些领域的创新和新应用。
