Wolf M B, Watson P D
Am J Physiol. 1985 Oct;249(4 Pt 2):H792-8. doi: 10.1152/ajpheart.1985.249.4.H792.
Capillary filtration coefficient (CFC) was measured in the isolated cat hindlimb preparation, perfused at 20 ml X min-1 X 100 g muscle-1 with a perfusate containing 6 g/dl albumin and normal electrolyte concentrations, to which were added 50 ml of the cat's blood and 6 micrograms of the vasodilator isoproterenol. CFC was determined three to six times in an initial control period during which the tissue temperature (measured by a 5-mm disk thermistor implanted in a thigh muscle) was controlled near 37 degrees C. Tissue temperature was decreased to 5-10 degrees C by lowering perfusate and ambient air temperatures. About 50 min were required for tissue temperature equilibration. CFC was measured at low temperature and then again at 37 degrees C. For nine experiments, the ratio of CFC at low temperature to that in the 37 degrees C control periods averaged 87% of the ratio of water viscosity at 37 degrees C to that at low temperature. The activation energy for water calculated from these data was 5.0 kcal/mol. These results may be explained by all transcapillary water flow moving by diffusion through narrow pores or by about 90% moving by convection, with the remainder going through a lipid pathway. However, the results may be entirely due to a direct effect of temperature on the geometry of the transcapillary pathway for water movement.
在分离的猫后肢标本中测量毛细血管滤过系数（CFC）。该标本以20 ml·min⁻¹·100 g肌肉⁻¹的灌注速率，用含有6 g/dl白蛋白和正常电解质浓度的灌注液进行灌注，并向其中加入50 ml猫血和6微克血管扩张剂异丙肾上腺素。在初始对照期内对CFC进行3至6次测定，在此期间，通过植入大腿肌肉中的5毫米圆盘热敏电阻测量的组织温度被控制在接近37℃。通过降低灌注液和环境空气温度将组织温度降至5 - 10℃。组织温度平衡大约需要50分钟。在低温下测量CFC，然后在37℃时再次测量。在九个实验中，低温下CFC与37℃对照期CFC的比值平均为37℃时水的粘度与低温时水的粘度比值的87%。根据这些数据计算出的水的活化能为5.0千卡/摩尔。这些结果可以解释为所有跨毛细血管水流通过扩散穿过狭窄孔隙移动，或者约90%通过对流移动，其余部分通过脂质途径移动。然而，这些结果可能完全是由于温度对水移动的跨毛细血管途径几何结构的直接影响。
