线粒体基因组中具有线性和环状结构的复杂DNA分子组合。

The complex DNA molecular combination with a linear and circular structure in mitochondrial genome.

作者信息

Li Qing, Sheng Wentao

机构信息

Department of Biological Technology, Nanchang Normal University, Nanchang, Jiangxi, China.

Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang, China.

出版信息

Front Plant Sci. 2025 May 29;16:1590173. doi: 10.3389/fpls.2025.1590173. eCollection 2025.

DOI:10.3389/fpls.2025.1590173

PMID:40510170

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12158950/

Abstract

BACKGROUND

is an endangered Magnoliaceae species, holding scientific, aesthetic, and economic value. But limited genetic research was reported. To better understand genomics, we aimed to assemble and analyze its mitochondrial genome.

RESULTS

has a branched structure, composing a linear and circular molecular structure with 428,449 bp and 126,869 bp, and GC contents of 47.51% and 47.38%. The total genome size is 555,318 bp, with GC content of 47.47%. A total of 68 genes were annotated, comprising 40 protein-coding, 23 tRNA, three rRNA genes, and two pseudo-genes. And , , and exhibited the highest nucleotide diversity, while and exhibited the lowest. Relative synonymous codon usage (RSCU) analysis revealed 29 codons with RSCU values > 1, showing A/U preference for codons ending. Moreover, 211 simple sequence, 1101 dispersed, and 39 tandem repeats were checked. The mitochondrial genome of and showed relatively good synteny. And 32 homologous fragments were discriminated between its mitochondrial and chloroplast genome, with a total of 29,253 bp and an average 914 bp. Phylogenetic analysis indicated that is the most closely related to .

CONCLUSION

The information provided herein contributes genomic knowledge for the breeding research.

摘要

背景

是木兰科一种濒危物种，具有科学、美学和经济价值。但已报道的遗传研究有限。为了更好地了解其基因组学，我们旨在组装并分析其线粒体基因组。

结果

具有分支结构，由一个428,449 bp的线性分子结构和一个126,869 bp的环状分子结构组成，GC含量分别为47.51%和47.38%。基因组总大小为555,318 bp，GC含量为47.47%。共注释了68个基因，包括40个蛋白质编码基因、23个tRNA基因、3个rRNA基因和2个假基因。并且，、和表现出最高的核苷酸多样性，而和表现出最低的核苷酸多样性。相对同义密码子使用（RSCU）分析显示29个密码子的RSCU值>1，表明以A/U结尾的密码子具有偏好性。此外，还检测到211个简单序列、1101个分散重复序列和39个串联重复序列。和的线粒体基因组显示出相对较好的共线性。并且在其线粒体和叶绿体基因组之间鉴别出32个同源片段，总长29,253 bp，平均长度914 bp。系统发育分析表明与关系最为密切。