Shenzhen Fairy Lake Botanical Garden, Guangdong, P.R. China.
Genome. 2011 Aug;54(8):663-73. doi: 10.1139/g11-026. Epub 2011 Jul 27.
Here, we report a completely sequenced plastome using Illumina/Solexa sequencing-by-synthesis (SBS) technology. The plastome of Magnolia kwangsiensis Figlar & Noot. is 159 667 bp in length with a typical quadripartite structure: 88 030 bp large single-copy (LSC) and 18 669 bp small single-copy (SSC) regions, separated by two 26 484 bp inverted repeat (IR) regions. The overall predicted gene number is 129, among which 17 genes are duplicated in IR regions. The plastome of M. kwangsiensis is identical in its gene order to previously published plastomes of magnoliids. Furthermore, the C-to-U type RNA editing frequency of 114 seed plants is positively correlated with plastome GC content and plastome length, whereas plastome length is not correlated with GC content. A total of 16 potential putative barcoding or low taxonomic level phylogenetic study markers in Magnoliaceae were detected by comparing the coding and noncoding regions of the plastome of M. kwangsiensis with that of Liriodendron tulipifera L. At least eight markers might be applied not only to Magnoliaceae but also to other taxa. The 86 mononucleotide cpSSRs that distributed in single-copy noncoding regions are highly valuable to study population genetics and conservation genetics of this endangered rare species.
在这里，我们使用 Illumina/Solexa 测序合成（SBS）技术报道了一个完整测序的质体基因组。广西木莲（Magnolia kwangsiensis Figlar & Noot.）的质体基因组长 159667bp，具有典型的四分体结构：88030bp 的大单拷贝（LSC）和 18669bp 的小单拷贝（SSC）区域，由两个 26484bp 的反向重复（IR）区域隔开。预测的基因总数为 129 个，其中 17 个基因在 IR 区域中重复。广西木莲的质体基因组在基因顺序上与已发表的木兰类质体基因组相同。此外，114 种种子植物的 C 到 U 型 RNA 编辑频率与质体 GC 含量和质体长度呈正相关，而质体长度与 GC 含量无关。通过比较广西木莲质体基因组与鹅掌楸（Liriodendron tulipifera L.）的编码区和非编码区，共检测到木兰科 16 个潜在的潜在条形码或低分类水平的系统发育研究标记。至少有 8 个标记不仅可以应用于木兰科，也可以应用于其他类群。分布在单拷贝非编码区的 86 个单核苷酸 cpSSR 对研究该濒危珍稀物种的种群遗传学和保护遗传学具有重要价值。
