Characterization of Cuproptosis-Related LncRNAs Prognostic Signature and Identification of LINC02285 as a Novel Biomarker for Ovarian Cancer.

BACKGROUND

Cuproptosis, a novel form of cell death triggered by copper ion accumulation, has shown potential in cancer therapy, particularly through the involvement of cuproptosis-related lnRNAs (CRLs). In-depth analyses exploring the relationship between CRLs and ovarian cancer (OC) are currently limited.

METHODS

LASSO-Cox regression analysis was conducted to assess the prognostic significance of CRLs and develop a cuproptosis-related prognostic model. Furthermore, tumor immune microenvironment and drug sensitivity were analyzed using ssGSEA, GSVA, ESTIMATE, and CIBERSORT algorithms. Finally, the tumorigenic effect of LINC02285 in OC and its correlation with cuproptosis were investigated by in vitro cell experiments.

RESULTS

We identified 138 CRLs correlated with cuproptosis-related genes (CRGs), 4 key CRLs (AC080038.1, AC083880.1, LINC00861, and LINC02285) were used to develop a prognostic model that effectively distinguished between low-risk and high-risk patients with significant differences in overall survival and progression-free survival. ROC curve analysis further validated the predictive capacity of the signature. Additionally, the low-risk group had a favorable prognosis associated with a protective immune microenvironment and a better response to targeted drugs. Conversely, the high-risk group displayed aggressive tumor features and poor immunotherapy outcomes. Validation through qPCR confirmed the differential expression of these CRLs in OC cells compared to normal ovarian cells, underscoring their potential significance in tumor biology. Bioinformatics analyses corroborated the association of LINC02285 with poor prognosis in OC patients. Functional experiment results showed that abnormal expression of LINC02285 could significantly regulate the proliferation and migration of OC cells. In addition, overexpression of LINC02285 markedly attenuated the inhibitory effects of Elesclomol-CuCl on OC cell activity.

CONCLUSION

Our study established a prognostic model based on 4 CRLs to assess overall survival and the immune microenvironment in OC patients. As a high-risk factor, LINC02285 demonstrates potential as a prognostic biomarker for OC.