Taechamaeteekul Preechaphon, Adi Yosua Kristian, Suwimonteerabutr Junpen, Sang-Gassanee Kridtasak, Lin Hongyao, Collell Miquel, Knox Robert V, Tummaruk Padet
Centre of Excellence in Swine Reproduction, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University Gadjah Mada, 5 Yogyakarta, Indonesia.
Theriogenology. 2025 Oct 1;245:117534. doi: 10.1016/j.theriogenology.2025.117534. Epub 2025 Jun 11.
Administering exogenous GnRH at the onset of standing estrus induces ovulation within ∼37.5 ± 3.3 h by stimulating LH secretion, narrowing the optimal insemination window. This study evaluated the effects of supplementing extended boar semen with a GnRH analog (buserelin) for fixed-time AI on conception rate, farrowing rate, and litter size under tropical field conditions. A total of 741 French Landrace × Yorkshire females (319 gilts, 422 sows) were used across nine replicates. After estrus detection, females were randomly assigned to four groups: control (n = 191), 10-IM (n = 183; 10 μg buserelin i.m.), 5-S (n = 181; semen + 5 μg), and 10-S (n = 186; semen + 10 μg). Gilts were inseminated at 0, 12, and 24 h; sows at 12, 24, and 36 h post-estrus. Litter data were collected from 223 farrowings: control (29 gilts, 34 sows), 10-IM (16 gilts, 35 sows), 5-S (29 gilts, 25 sows), and 10-S (27 gilts, 28 sows). Across groups, average farrowing rate was 88.9 %, with total number of piglets born per litter (TB) 17.0 ± 4.0, number of piglets born alive per litter (BA) 15.2 ± 3.5, stillborn 1.0 ± 1.5, and mummified fetuses 0.5 ± 1.2. No significant differences in conception or farrowing rates were found. The 5-S group had a significantly higher litter weight than the control group (24.5 ± 2.0 kg vs. 19.4 ± 0.7 kg; P = 0.018). Gilts receiving 5 μg buserelin in semen had significantly higher TB, BA, and litter weight (P < 0.05), while 10 μg increased litter weight without affecting TB. In sows, no significant improvements were observed. These results suggest that supplementing semen with low-dose GnRH analog can improve gilt productivity under tropical conditions and merits further investigation.
在静立发情开始时注射外源性促性腺激素释放激素（GnRH），通过刺激促黄体生成素（LH）分泌，在约37.5±3.3小时内诱导排卵，从而缩小最佳授精窗口。本研究评估了在热带田间条件下，在延长的公猪精液中添加GnRH类似物（布舍瑞林）进行定时人工授精对受胎率、产仔率和窝仔数的影响。在9个重复试验中总共使用了741头法国长白猪×约克夏母猪（319头后备母猪，422头经产母猪）。发情检测后，将母猪随机分为四组：对照组（n = 191）、10-IM组（n = 183；肌肉注射10μg布舍瑞林）、5-S组（n = 181；精液+5μg）和10-S组（n = 186；精液+10μg）。后备母猪在发情后0、12和24小时进行授精；经产母猪在发情后12、24和36小时进行授精。从223窝产仔中收集窝仔数据：对照组（29头后备母猪，34头经产母猪）、10-IM组（16头后备母猪，35头经产母猪）、5-S组（29头后备母猪，25头经产母猪）和10-S组（27头后备母猪，28头经产母猪）。各组平均产仔率为88.9%，每窝出生仔猪总数（TB）为17.0±4.0头，每窝活产仔猪数（BA）为15.2±3.5头，死胎1.0±1.5头，木乃伊胎0.5±1.2头。受胎率和产仔率未发现显著差异。5-S组的窝重显著高于对照组（24.5±2.0千克对19.4±0.7千克；P = 0.018）。在精液中添加5μg布舍瑞林的后备母猪的TB、BA和窝重显著更高（P < 0.05），而添加10μg可增加窝重但不影响TB。在经产母猪中，未观察到显著改善。这些结果表明，在热带条件下，在精液中添加低剂量GnRH类似物可提高后备母猪的繁殖性能，值得进一步研究。
