Karimi-Rastehkenari Amin, Youseftabar-Miri Leila, Askarizadeh Elham, Divsar Faten
Active Pharmaceutical Ingredients Research Center (APIRC), Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Int J Biol Macromol. 2025 Aug;319(Pt 4):145211. doi: 10.1016/j.ijbiomac.2025.145211. Epub 2025 Jun 11.
This work focuses on preparing novel FeO magnetic nanoparticles (MNPs) imprinted with Chitosan/Hyaluronic acid (Cs/HA) hybrid hydrogels via an in situ technique, presenting a Cs/HA/Fe3O4 nanocomposite hydrogel for the in vitro release of the ciprofloxacin (CFX) antibiotic drug. The properties of the prepared nanocomposite hydrogel have been explored using Fourier-Transform infrared spectrometric (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Energy dispersive X-ray (EDX) mapping, Transition Electron Microscopic (TEM), Vibrating-sample magnetometer (VSM), X-ray diffractometric (XRD), Thermogravimetric analysis (TGA), Ultra Violet- Visible (UV-Vis), rheological, swelling, and drug release studies. Notably, the distribution of nanosized FeO MNPs within the Cs/HA hydrogel scaffold was found to be uniformly dispersed, indicating a well-controlled synthesis process. Indeed, the presence of FeO MNPs increased the swelling rate of the hydrogel. The pH-responsive Cs/HA/FeO nanocomposite hydrogel enabled the sustained release of CFX, with 93 % entrapment efficiency and 100 % release within 13 h at various pH levels. The drug-loaded hydrogel demonstrated a noteworthy zone of inhibition against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The analysis of cell toxicity percentages at specified concentrations, along with the half-maximal inhibitory concentration (IC50), suggests that the Cs/HA/FeO hydrogel demonstrates a favorable safety profile.
本研究聚焦于通过原位技术制备用壳聚糖/透明质酸（Cs/HA）杂化水凝胶印迹的新型FeO磁性纳米颗粒（MNP），呈现一种用于环丙沙星（CFX）抗生素药物体外释放的Cs/HA/Fe₃O₄纳米复合水凝胶。已使用傅里叶变换红外光谱（FT-IR）、场发射扫描电子显微镜（FE-SEM）、能量色散X射线（EDX）映射、透射电子显微镜（TEM）、振动样品磁强计（VSM）、X射线衍射（XRD）、热重分析（TGA）、紫外可见（UV-Vis）、流变学、溶胀和药物释放研究等方法对制备的纳米复合水凝胶的性能进行了探究。值得注意的是，发现纳米尺寸的FeO MNP在Cs/HA水凝胶支架内均匀分散，表明合成过程得到了良好控制。事实上，FeO MNP的存在提高了水凝胶的溶胀率。pH响应性Cs/HA/FeO纳米复合水凝胶能够实现CFX的持续释放，在不同pH水平下包封率为93%，13小时内释放率为100%。载药水凝胶对革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌均表现出显著的抑菌圈。在特定浓度下对细胞毒性百分比以及半数最大抑制浓度（IC50）的分析表明，Cs/HA/FeO水凝胶具有良好的安全性。
