使用羧甲基纤维素/聚（丙烯酸-衣康酸）/Cloisite 30B 纳米复合水凝胶作为 pH 响应载体口服递送马来酸舒尼替尼。

Oral delivery of Sunitinib malate using carboxymethyl cellulose/poly(acrylic acid-itaconic acid)/Cloisite 30B nanocomposite hydrogel as a pH-responsive carrier.

机构信息

Department of Chemical Engineering, University of Bonab, Bonab, 55513-95133, Iran.

Faculty of Chemical and Petroleum Engineering, University of Tabriz, Tabriz, 5166616471, Iran.

出版信息

BMC Biotechnol. 2024 Sep 30;24(1):70. doi: 10.1186/s12896-024-00883-0.

This work aimed to fabricate a Cloisite 30B-incorporated carboxymethyl cellulose graft copolymer of acrylic acid and itaconic acid hydrogel (Hyd) via a free radical polymerization method for controlled release of Sunitinib malate anticancer drug. The synthesized samples were characterized by FTIR, XRD, TEM, and SEM-dot mapping analyses. The encapsulation efficiency of Hyd and Hyd/Cloisite 30B (6 wt%) was 81 and 93%, respectively, showing the effectiveness of Cloisite 30B in drug loading. An in vitro drug release study showed that drug release from all samples in a buffer solution with pH 7.4 was higher than in a buffer solution with pH 5.5. During 240 min, the cumulative drug release from Hyd/Cloisite 30B (94.97% at pH 7.4) is lower than Hyd (53.71% at pH 7.4). Also, drug-loaded Hyd/Cloisite 30B (6 wt%) demonstrated better antibacterial activity towards S. Aureus bacteria and E. Coli. High anticancer activity of Hyd/Cloisite 30B against MCF-7 human breast cancer cells was shown by the MTT assay, with a MCF-7 cell viability of 23.82 ± 1.23% after 72-hour incubation. Our results suggest that Hyd/Cloisite 30B could be used as a pH-controlled carrier to deliver anticancer Sunitinib malate.

本工作旨在通过自由基聚合方法制备蒙脱土 30B 复合羧甲基纤维素接枝共聚物丙烯酸和衣康酸水凝胶（Hyd），用于马来酸舒尼替尼抗癌药物的控制释放。通过 FTIR、XRD、TEM 和 SEM 点映射分析对合成样品进行了表征。Hyd 和 Hyd/Cloisite 30B（6wt%）的包封效率分别为 81%和 93%，表明 Cloisite 30B 对药物负载有效。体外药物释放研究表明，所有样品在 pH 值为 7.4 的缓冲溶液中的药物释放率均高于在 pH 值为 5.5 的缓冲溶液中的药物释放率。在 240 分钟内，Hyd/Cloisite 30B（pH 值为 7.4 时为 94.97%）的累积药物释放量低于 Hyd（pH 值为 7.4 时为 53.71%）。此外，负载药物的 Hyd/Cloisite 30B（6wt%）对金黄色葡萄球菌和大肠杆菌表现出更好的抗菌活性。MTT 测定法显示，Hyd/Cloisite 30B 对 MCF-7 人乳腺癌细胞具有高抗癌活性，在 72 小时孵育后 MCF-7 细胞活力为 23.82±1.23%。我们的结果表明，Hyd/Cloisite 30B 可用作 pH 控制载体，用于输送抗癌药物舒尼替尼马来酸盐。